6d08: Difference between revisions
New page: '''Unreleased structure''' The entry 6d08 is ON HOLD until Apr 10 2020 Authors: Description: Category: Unreleased Structures |
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==Crystal structure of an engineered bump-hole complex of mutant human chromobox homolog 1 (CBX1) with H3K9bn peptide== | |||
<StructureSection load='6d08' size='340' side='right'caption='[[6d08]], [[Resolution|resolution]] 2.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6d08]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D08 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D08 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FQA:N~6~-benzyl-L-lysine'>FQA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d08 OCA], [https://pdbe.org/6d08 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d08 RCSB], [https://www.ebi.ac.uk/pdbsum/6d08 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d08 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/CBX1_HUMAN CBX1_HUMAN] Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Protein-protein interactions mediated by methyllysine are ubiquitous in biological systems. Specific perturbation of such interactions has remained a challenging endeavor. Herein, we describe an allele-specific strategy toward an engineered protein-protein interface orthogonal to the human proteome. We develop a methyltransferase (writer) variant that installs aryllysine moiety on histones that can only be recognized by an engineered chromodomain (reader). We establish biochemical integrity of the engineered interface, provide structural evidence for orthogonality and validate its applicability to identify transcriptional regulators. Our approach provides an unprecedented strategy for specific manipulation of the methyllysine interactome. | |||
Engineering Methyllysine Writers and Readers for Allele-Specific Regulation of Protein-Protein Interactions.,Arora S, Horne WS, Islam K J Am Chem Soc. 2019 Oct 2;141(39):15466-15470. doi: 10.1021/jacs.9b05725. Epub, 2019 Sep 20. PMID:31518125<ref>PMID:31518125</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6d08" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Arora S]] | |||
[[Category: Horne WS]] | |||
[[Category: Islam K]] | |||
Latest revision as of 18:14, 4 October 2023
Crystal structure of an engineered bump-hole complex of mutant human chromobox homolog 1 (CBX1) with H3K9bn peptideCrystal structure of an engineered bump-hole complex of mutant human chromobox homolog 1 (CBX1) with H3K9bn peptide
Structural highlights
FunctionCBX1_HUMAN Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane. Publication Abstract from PubMedProtein-protein interactions mediated by methyllysine are ubiquitous in biological systems. Specific perturbation of such interactions has remained a challenging endeavor. Herein, we describe an allele-specific strategy toward an engineered protein-protein interface orthogonal to the human proteome. We develop a methyltransferase (writer) variant that installs aryllysine moiety on histones that can only be recognized by an engineered chromodomain (reader). We establish biochemical integrity of the engineered interface, provide structural evidence for orthogonality and validate its applicability to identify transcriptional regulators. Our approach provides an unprecedented strategy for specific manipulation of the methyllysine interactome. Engineering Methyllysine Writers and Readers for Allele-Specific Regulation of Protein-Protein Interactions.,Arora S, Horne WS, Islam K J Am Chem Soc. 2019 Oct 2;141(39):15466-15470. doi: 10.1021/jacs.9b05725. Epub, 2019 Sep 20. PMID:31518125[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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