6d08
Crystal structure of an engineered bump-hole complex of mutant human chromobox homolog 1 (CBX1) with H3K9bn peptideCrystal structure of an engineered bump-hole complex of mutant human chromobox homolog 1 (CBX1) with H3K9bn peptide
Structural highlights
FunctionCBX1_HUMAN Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane. Publication Abstract from PubMedProtein-protein interactions mediated by methyllysine are ubiquitous in biological systems. Specific perturbation of such interactions has remained a challenging endeavor. Herein, we describe an allele-specific strategy toward an engineered protein-protein interface orthogonal to the human proteome. We develop a methyltransferase (writer) variant that installs aryllysine moiety on histones that can only be recognized by an engineered chromodomain (reader). We establish biochemical integrity of the engineered interface, provide structural evidence for orthogonality and validate its applicability to identify transcriptional regulators. Our approach provides an unprecedented strategy for specific manipulation of the methyllysine interactome. Engineering Methyllysine Writers and Readers for Allele-Specific Regulation of Protein-Protein Interactions.,Arora S, Horne WS, Islam K J Am Chem Soc. 2019 Oct 2;141(39):15466-15470. doi: 10.1021/jacs.9b05725. Epub, 2019 Sep 20. PMID:31518125[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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