5tud: Difference between revisions
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==Structural Insights into the Extracellular Recognition of the Human Serotonin 2B Receptor by an Antibody== | ==Structural Insights into the Extracellular Recognition of the Human Serotonin 2B Receptor by an Antibody== | ||
<StructureSection load='5tud' size='340' side='right' caption='[[5tud]], [[Resolution|resolution]] 3.00Å' scene=''> | <StructureSection load='5tud' size='340' side='right'caption='[[5tud]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5tud]] is a 6 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5tud]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TUD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TUD FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ERM:ERGOTAMINE'>ERM</scene></td></tr> | |||
<tr id=' | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tud FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tud OCA], [https://pdbe.org/5tud PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tud RCSB], [https://www.ebi.ac.uk/pdbsum/5tud PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tud ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[[ | [https://www.uniprot.org/uniprot/C562_ECOLX C562_ECOLX] Electron-transport protein of unknown function.[https://www.uniprot.org/uniprot/5HT2B_HUMAN 5HT2B_HUMAN] This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Plays a role in the regulation of impulsive behavior. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 22: | Line 21: | ||
==See Also== | ==See Also== | ||
*[[5-hydroxytryptamine receptor|5-hydroxytryptamine receptor]] | *[[5-hydroxytryptamine receptor 3D structures|5-hydroxytryptamine receptor 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia coli]] | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: Basu | [[Category: Large Structures]] | ||
[[Category: Boutet | [[Category: Mus musculus]] | ||
[[Category: Cherezov | [[Category: Basu S]] | ||
[[Category: Han | [[Category: Boutet S]] | ||
[[Category: Ishchenko | [[Category: Cherezov V]] | ||
[[Category: James | [[Category: Han GW]] | ||
[[Category: Kapoor | [[Category: Ishchenko A]] | ||
[[Category: Katritch | [[Category: James D]] | ||
[[Category: Liu | [[Category: Kapoor M]] | ||
[[Category: Stevens | [[Category: Katritch V]] | ||
[[Category: Wacker | [[Category: Liu W]] | ||
[[Category: Wang | [[Category: Stevens RC]] | ||
[[Category: Weierstall | [[Category: Wacker D]] | ||
[[Category: Zhang | [[Category: Wang D]] | ||
[[Category: Weierstall U]] | |||
[[Category: Zhang A]] | |||
Latest revision as of 16:12, 4 October 2023
Structural Insights into the Extracellular Recognition of the Human Serotonin 2B Receptor by an AntibodyStructural Insights into the Extracellular Recognition of the Human Serotonin 2B Receptor by an Antibody
Structural highlights
FunctionC562_ECOLX Electron-transport protein of unknown function.5HT2B_HUMAN This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Plays a role in the regulation of impulsive behavior. Publication Abstract from PubMedMonoclonal antibodies provide an attractive alternative to small-molecule therapies for a wide range of diseases. Given the importance of G protein-coupled receptors (GPCRs) as pharmaceutical targets, there has been an immense interest in developing therapeutic monoclonal antibodies that act on GPCRs. Here we present the 3.0-A resolution structure of a complex between the human 5-hydroxytryptamine 2B (5-HT2B) receptor and an antibody Fab fragment bound to the extracellular side of the receptor, determined by serial femtosecond crystallography with an X-ray free-electron laser. The antibody binds to a 3D epitope of the receptor that includes all three extracellular loops. The 5-HT2B receptor is captured in a well-defined active-like state, most likely stabilized by the crystal lattice. The structure of the complex sheds light on the mechanism of selectivity in extracellular recognition of GPCRs by monoclonal antibodies. Structural insights into the extracellular recognition of the human serotonin 2B receptor by an antibody.,Ishchenko A, Wacker D, Kapoor M, Zhang A, Han GW, Basu S, Patel N, Messerschmidt M, Weierstall U, Liu W, Katritch V, Roth BL, Stevens RC, Cherezov V Proc Natl Acad Sci U S A. 2017 Aug 1;114(31):8223-8228. doi:, 10.1073/pnas.1700891114. Epub 2017 Jul 17. PMID:28716900[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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