4rw3: Difference between revisions
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==Structural insights into substrate binding of brown spider venom class II phospholipases D== | ==Structural insights into substrate binding of brown spider venom class II phospholipases D== | ||
<StructureSection load='4rw3' size='340' side='right' caption='[[4rw3]], [[Resolution|resolution]] 1.72Å' scene=''> | <StructureSection load='4rw3' size='340' side='right'caption='[[4rw3]], [[Resolution|resolution]] 1.72Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4rw3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RW3 OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[4rw3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Loxosceles_intermedia Loxosceles intermedia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RW3 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DKA:DECANOIC+ACID'>DKA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPD:D-MYO-INOSITOL-1-PHOSPHATE'>IPD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OCA:OCTANOIC+ACID+(CAPRYLIC+ACID)'>OCA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=SHV:HEPTANOIC+ACID'>SHV</scene>, <scene name='pdbligand=TDA:N-TRIDECANOIC+ACID'>TDA</scene | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.72Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DKA:DECANOIC+ACID'>DKA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPD:D-MYO-INOSITOL-1-PHOSPHATE'>IPD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OCA:OCTANOIC+ACID+(CAPRYLIC+ACID)'>OCA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=SHV:HEPTANOIC+ACID'>SHV</scene>, <scene name='pdbligand=TDA:N-TRIDECANOIC+ACID'>TDA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rw3 OCA], [https://pdbe.org/4rw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rw3 RCSB], [https://www.ebi.ac.uk/pdbsum/4rw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rw3 ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/A1HB2_LOXIN A1HB2_LOXIN] Catalyzes the hydrolysis of sphingomyelin. May also acts on other phosphatidyl esters. Induces complement-dependent hemolysis, dermonecrosis, blood vessel permeability and platelet aggregation.<ref>PMID:9790962</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 19: | Line 19: | ||
</div> | </div> | ||
<div class="pdbe-citations 4rw3" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 4rw3" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Phospholipase D 3D structures|Phospholipase D 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Arni | [[Category: Loxosceles intermedia]] | ||
[[Category: Chahine | [[Category: Arni RK]] | ||
[[Category: Chaim | [[Category: Chahine J]] | ||
[[Category: Chaves-Moreira | [[Category: Chaim OM]] | ||
[[Category: Coronado | [[Category: Chaves-Moreira D]] | ||
[[Category: Murakami | [[Category: Coronado MA]] | ||
[[Category: Murakami MT]] | |||
[[Category: Ullah | [[Category: Ullah A]] | ||
[[Category: Veiga | [[Category: Veiga SS]] | ||
[[Category: Vuitika | [[Category: Vuitika L]] | ||
[[Category: | [[Category: Da Silva LS]] | ||
Latest revision as of 20:57, 20 September 2023
Structural insights into substrate binding of brown spider venom class II phospholipases DStructural insights into substrate binding of brown spider venom class II phospholipases D
Structural highlights
FunctionA1HB2_LOXIN Catalyzes the hydrolysis of sphingomyelin. May also acts on other phosphatidyl esters. Induces complement-dependent hemolysis, dermonecrosis, blood vessel permeability and platelet aggregation.[1] Publication Abstract from PubMedPhospholipases D (PLDs) the major dermonecrotic factors from brown spider venoms trigger a range of biological reactions both in vitro and in vivo. Despite their clinical relevance in loxoscelism, structural data is restricted to the apo-form of these enzymes, which has been instrumental in understanding the functional differences between the class I and II spider PLDs. The crystal structures of the native class II PLD from Loxosceles intermedia complexed with myo-inositol 1-phosphate and the inactive mutant H12A complexed with fatty acids indicate the existence of a strong ligand-dependent conformation change of the highly conserved aromatic residues, Tyr 223 and Trp225 indicating their roles in substrate binding. These results provided insights into the structural determinants for substrate recognition and binding by class II PLDs. Structural Insights into Substrate Binding of Brown Spider Venom Class II Phospholipases D.,Coronado MA, Ullah A, da Silva LS, Chaves-Moreira D, Vuitika L, Chaim OM, Veiga SS, Chahine J, Murakami MT, Arni RK Curr Protein Pept Sci. 2015 May 5. PMID:25961401[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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