4rw3: Difference between revisions

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-'''Unreleased structure'''
-The entry 4rw3 is ON HOLD
+==Structural insights into substrate binding of brown spider venom class II phospholipases D==
+<StructureSection load='4rw3' size='340' side='right'caption='[[4rw3]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4rw3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Loxosceles_intermedia Loxosceles intermedia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RW3 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.72&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DKA:DECANOIC+ACID'>DKA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPD:D-MYO-INOSITOL-1-PHOSPHATE'>IPD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OCA:OCTANOIC+ACID+(CAPRYLIC+ACID)'>OCA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=SHV:HEPTANOIC+ACID'>SHV</scene>, <scene name='pdbligand=TDA:N-TRIDECANOIC+ACID'>TDA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rw3 OCA], [https://pdbe.org/4rw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rw3 RCSB], [https://www.ebi.ac.uk/pdbsum/4rw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rw3 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A1HB2_LOXIN A1HB2_LOXIN] Catalyzes the hydrolysis of sphingomyelin. May also acts on other phosphatidyl esters. Induces complement-dependent hemolysis, dermonecrosis, blood vessel permeability and platelet aggregation.<ref>PMID:9790962</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Phospholipases D (PLDs) the major dermonecrotic factors from brown spider venoms trigger a range of biological reactions both in vitro and in vivo. Despite their clinical relevance in loxoscelism, structural data is restricted to the apo-form of these enzymes, which has been instrumental in understanding the functional differences between the class I and II spider PLDs. The crystal structures of the native class II PLD from Loxosceles intermedia complexed with myo-inositol 1-phosphate and the inactive mutant H12A complexed with fatty acids indicate the existence of a strong ligand-dependent conformation change of the highly conserved aromatic residues, Tyr 223 and Trp225 indicating their roles in substrate binding. These results provided insights into the structural determinants for substrate recognition and binding by class II PLDs.
-Authors: Coronado, M.A., Ullah, A., da Silva, L.S., Chaves-Moreira, D., Vuitika, L., Chaim, O.M., Veiga, S.S., Chahine, J., Murakami, M.T., Arni, R.K.
+Structural Insights into Substrate Binding of Brown Spider Venom Class II Phospholipases D.,Coronado MA, Ullah A, da Silva LS, Chaves-Moreira D, Vuitika L, Chaim OM, Veiga SS, Chahine J, Murakami MT, Arni RK Curr Protein Pept Sci. 2015 May 5. PMID:25961401<ref>PMID:25961401</ref>
-Description: Structural insights into substrate binding of brown spider venom class II phospholipases D
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Coronado, M.A]]
+<div class="pdbe-citations 4rw3" style="background-color:#fffaf0;"></div>
-[[Category: Vuitika, L]]
-[[Category: Chaves-Moreira, D]]
+==See Also==
-[[Category: Arni, R.K]]
+*[[Phospholipase D 3D structures|Phospholipase D 3D structures]]
-[[Category: Chahine, J]]
+== References ==
-[[Category: Chaim, O.M]]
+<references/>
-[[Category: Ullah, A]]
+__TOC__
-[[Category: Da Silva, L.S]]
+</StructureSection>
-[[Category: Murakami, M.T]]
+[[Category: Large Structures]]
-[[Category: Veiga, S.S]]
+[[Category: Loxosceles intermedia]]
+[[Category: Arni RK]]
+[[Category: Chahine J]]
+[[Category: Chaim OM]]
+[[Category: Chaves-Moreira D]]
+[[Category: Coronado MA]]
+[[Category: Murakami MT]]
+[[Category: Ullah A]]
+[[Category: Veiga SS]]
+[[Category: Vuitika L]]
+[[Category: Da Silva LS]]