3o0e: Difference between revisions

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-[[Image:3o0e.png|left|200px]]
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+==Crystal structure of OmpF in complex with colicin peptide OBS1==
-The line below this paragraph, containing "STRUCTURE_3o0e", creates the "Structure Box" on the page.
+<StructureSection load='3o0e' size='340' side='right'caption='[[3o0e]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3o0e]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O0E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O0E FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.01&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr>
-{{STRUCTURE_3o0e|  PDB=3o0e  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o0e OCA], [https://pdbe.org/3o0e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o0e RCSB], [https://www.ebi.ac.uk/pdbsum/3o0e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o0e ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/OMPF_ECOLI OMPF_ECOLI] Forms pores that allow passive diffusion of small molecules across the outer membrane. It is also a receptor for the bacteriophage T2.<ref>PMID:19721064</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The porins OmpF and OmpC are trimeric beta-barrel proteins with narrow channels running through each monomer that exclude molecules &gt; 600 Da while mediating the passive diffusion of small nutrients and metabolites across the Gram-negative outer membrane (OM). Here, we elucidate the mechanism by which an entire soluble protein domain (&gt; 6 kDa) is delivered through the lumen of such porins. Following high-affinity binding to the vitamin B(12) receptor in Escherichia coli, the bacteriocin ColE9 recruits OmpF or OmpC using an 83-residue intrinsically unstructured translocation domain (IUTD) to deliver a 16-residue TolB-binding epitope (TBE) in the center of the IUTD to the periplasm where it triggers toxin entry. We demonstrate that the IUTD houses two OmpF-binding sites, OBS1 (residues 2-18) and OBS2 (residues 54-63), which flank the TBE and bind with K(d)s of 2 and 24 muM, respectively, at pH 6.5 and 25 masculineC. We show the two OBSs share the same binding site on OmpF and that the colicin must house at least one of them for antibiotic activity. Finally, we report the structure of the OmpF-OBS1 complex that shows the colicin bound within the porin lumen spanning the membrane bilayer. Our study explains how colicins exploit porins to deliver epitope signals to the bacterial periplasm and, more broadly, how the inherent flexibility and narrow cross-sectional area of an IUP domain can endow it with the ability to traverse a biological membrane via the constricted lumen of a beta-barrel membrane protein.
-===Crystal structure of OmpF in complex with colicin peptide OBS1===
+Directed epitope delivery across the Escherichia coli outer membrane through the porin OmpF.,Housden NG, Wojdyla JA, Korczynska J, Grishkovskaya I, Kirkpatrick N, Brzozowski AM, Kleanthous C Proc Natl Acad Sci U S A. 2010 Nov 22. PMID:21098297<ref>PMID:21098297</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_21098297}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 3o0e" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 21098297 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_21098297}}
-==About this Structure==
-[[3o0e]] is a 12 chain structure of [[Porin]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O0E OCA].
 ==See Also==
-*[[OmpF]]
+*[[Colicin 3D structures|Colicin 3D structures]]
-*[[Porin]]
+*[[Porin 3D structures|Porin 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:21098297</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Escherichia coli]]
-[[Category: Brzozowski, A M.]]
+[[Category: Large Structures]]
-[[Category: Grishkovskaya, I.]]
+[[Category: Brzozowski AM]]
-[[Category: Housden, N G.]]
+[[Category: Grishkovskaya I]]
-[[Category: Kirkpatrick, N.]]
+[[Category: Housden NG]]
-[[Category: Kleanthous, C.]]
+[[Category: Kirkpatrick N]]
-[[Category: Korczynska, J.]]
+[[Category: Kleanthous C]]
-[[Category: Wojdyla, J A.]]
+[[Category: Korczynska J]]
+[[Category: Wojdyla JA]]