3c4h: Difference between revisions

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[[Image:3c4h.png|left|200px]]


{{STRUCTURE_3c4h| PDB=3c4h | SCENE= }}
==Human poly(ADP-ribose) polymerase 3, catalytic fragment in complex with an inhibitor DR2313==
<StructureSection load='3c4h' size='340' side='right'caption='[[3c4h]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3c4h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C4H FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=DRL:2-METHYL-3,5,7,8-TETRAHYDRO-4H-THIOPYRANO[4,3-D]PYRIMIDIN-4-ONE'>DRL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c4h OCA], [https://pdbe.org/3c4h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c4h RCSB], [https://www.ebi.ac.uk/pdbsum/3c4h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c4h ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PARP3_HUMAN PARP3_HUMAN] Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. Binds DNA. May be involved in the regulation of PRC2 and PRC3 complex-dependent gene silencing.<ref>PMID:16924674</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c4/3c4h_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3c4h ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Poly(ADP-ribose) polymerases (PARPs) activate DNA repair mechanisms upon stress- and cytotoxin-induced DNA damage, and inhibition of PARP activity is a lead in cancer drug therapy. We present a structural and functional analysis of the PARP domain of human PARP-3 in complex with several inhibitors. Of these, KU0058948 is the strongest inhibitor of PARP-3 activity. The presented crystal structures highlight key features for potent inhibitor binding and suggest routes for creating isoenzyme-specific PARP inhibitors.


===Human poly(ADP-ribose) polymerase 3, catalytic fragment in complex with an inhibitor DR2313===
Structural basis for inhibitor specificity in human poly(ADP-ribose) polymerase-3.,Lehtio L, Jemth AS, Collins R, Loseva O, Johansson A, Markova N, Hammarstrom M, Flores A, Holmberg-Schiavone L, Weigelt J, Helleday T, Schuler H, Karlberg T J Med Chem. 2009 May 14;52(9):3108-11. PMID:19354255<ref>PMID:19354255</ref>


{{ABSTRACT_PUBMED_19354255}}
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
==About this Structure==
<div class="pdbe-citations 3c4h" style="background-color:#fffaf0;"></div>
[[3c4h]] is a 1 chain structure of [[Poly (ADP-ribose) polymerase]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C4H OCA].


==See Also==
==See Also==
*[[Poly (ADP-ribose) polymerase|Poly (ADP-ribose) polymerase]]
*[[Poly(ADP-ribose) polymerase 3D structures|Poly(ADP-ribose) polymerase 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:019354255</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Arrowsmith, C H.]]
[[Category: Large Structures]]
[[Category: Berg, S Van den.]]
[[Category: Arrowsmith CH]]
[[Category: Berglund, H.]]
[[Category: Berglund H]]
[[Category: Bountra, C.]]
[[Category: Bountra C]]
[[Category: Busam, R.]]
[[Category: Busam R]]
[[Category: Collins, R.]]
[[Category: Collins R]]
[[Category: Dahlgren, L G.]]
[[Category: Dahlgren LG]]
[[Category: Edwards, A M.]]
[[Category: Edwards AM]]
[[Category: Flodin, S.]]
[[Category: Flodin S]]
[[Category: Flores, A.]]
[[Category: Flores A]]
[[Category: Graslund, S.]]
[[Category: Graslund S]]
[[Category: Hammarstrom, M.]]
[[Category: Hammarstrom M]]
[[Category: Herman, M D.]]
[[Category: Herman MD]]
[[Category: Johansson, A.]]
[[Category: Johansson A]]
[[Category: Johansson, I.]]
[[Category: Johansson I]]
[[Category: Kallas, A.]]
[[Category: Kallas A]]
[[Category: Karlberg, T.]]
[[Category: Karlberg T]]
[[Category: Kotenyova, T.]]
[[Category: Kotenyova T]]
[[Category: Lehtio, L.]]
[[Category: Lehtio L]]
[[Category: Moche, M.]]
[[Category: Moche M]]
[[Category: Nilsson, M E.]]
[[Category: Nilsson ME]]
[[Category: Nordlund, P.]]
[[Category: Nordlund P]]
[[Category: Nyman, T.]]
[[Category: Nyman T]]
[[Category: Persson, C.]]
[[Category: Persson C]]
[[Category: SGC, Structural Genomics Consortium.]]
[[Category: Sagemark J]]
[[Category: Sagemark, J.]]
[[Category: Svensson L]]
[[Category: Svensson, L.]]
[[Category: Thorsell AG]]
[[Category: Thorsell, A G.]]
[[Category: Tresaugues L]]
[[Category: Tresaugues, L.]]
[[Category: Van den Berg S]]
[[Category: Weigelt, J.]]
[[Category: Weigelt J]]
[[Category: Welin, M.]]
[[Category: Welin M]]
[[Category: Catalytic fragment]]
[[Category: Enzyme-inhibitor complex]]
[[Category: Glycosyltransferase]]
[[Category: Nad]]
[[Category: Nucleus]]
[[Category: Sgc]]
[[Category: Structural genomic]]
[[Category: Structural genomics consortium]]
[[Category: Transferase]]

Latest revision as of 15:22, 30 August 2023

Human poly(ADP-ribose) polymerase 3, catalytic fragment in complex with an inhibitor DR2313Human poly(ADP-ribose) polymerase 3, catalytic fragment in complex with an inhibitor DR2313

Structural highlights

3c4h is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PARP3_HUMAN Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. Binds DNA. May be involved in the regulation of PRC2 and PRC3 complex-dependent gene silencing.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Poly(ADP-ribose) polymerases (PARPs) activate DNA repair mechanisms upon stress- and cytotoxin-induced DNA damage, and inhibition of PARP activity is a lead in cancer drug therapy. We present a structural and functional analysis of the PARP domain of human PARP-3 in complex with several inhibitors. Of these, KU0058948 is the strongest inhibitor of PARP-3 activity. The presented crystal structures highlight key features for potent inhibitor binding and suggest routes for creating isoenzyme-specific PARP inhibitors.

Structural basis for inhibitor specificity in human poly(ADP-ribose) polymerase-3.,Lehtio L, Jemth AS, Collins R, Loseva O, Johansson A, Markova N, Hammarstrom M, Flores A, Holmberg-Schiavone L, Weigelt J, Helleday T, Schuler H, Karlberg T J Med Chem. 2009 May 14;52(9):3108-11. PMID:19354255[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Rouleau M, McDonald D, Gagne P, Ouellet ME, Droit A, Hunter JM, Dutertre S, Prigent C, Hendzel MJ, Poirier GG. PARP-3 associates with polycomb group bodies and with components of the DNA damage repair machinery. J Cell Biochem. 2007 Feb 1;100(2):385-401. PMID:16924674 doi:10.1002/jcb.21051
  2. ↑ Lehtio L, Jemth AS, Collins R, Loseva O, Johansson A, Markova N, Hammarstrom M, Flores A, Holmberg-Schiavone L, Weigelt J, Helleday T, Schuler H, Karlberg T. Structural basis for inhibitor specificity in human poly(ADP-ribose) polymerase-3. J Med Chem. 2009 May 14;52(9):3108-11. PMID:19354255 doi:10.1021/jm900052j

3c4h, resolution 2.10Å

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