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== | ==WILD-TYPE PLASTOCYANIN FROM SILENE== | ||
Plastocyanin functions as an electron carrier between the cytochrome b6f | <StructureSection load='1byo' size='340' side='right'caption='[[1byo]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1byo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Silene_latifolia_subsp._alba Silene latifolia subsp. alba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BYO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BYO FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1byo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1byo OCA], [https://pdbe.org/1byo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1byo RCSB], [https://www.ebi.ac.uk/pdbsum/1byo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1byo ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PLAS_SILLB PLAS_SILLB] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.<ref>PMID:10220581</ref> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/by/1byo_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1byo ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Plastocyanin functions as an electron carrier between the cytochrome b6f complex and photosystem I. The crystal structures of the wild-type and E43K/D44K double mutant from the higher plant, Silene, have been determined at 2.0 and 1.75 A resolution, respectively. The wild-type plastocyanin comprises two monomers per asymmetric unit, one of which shows the unusually great distance between the copper ion and the Ndelta1 atom of H87 because of the hydrogen bond network formation between H87 and symmetry-related G10. The root mean square deviation for Ca atoms between the wild-type and mutant plastocyanins is 0.44 A, however, the electrostatic potential maps of their molecular surfaces are remarkably different. The low electron-transfer rate in the E43K/D44K mutant results from the hindrance of electrostatic interactions, not from the structural change due to the mutation. | |||
Crystal structures of wild-type and mutant plastocyanins from a higher plant, Silene.,Sugawara H, Inoue T, Li C, Gotowda M, Hibino T, Takabe T, Kai Y J Biochem. 1999 May;125(5):899-903. PMID:10220581<ref>PMID:10220581</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
<div class="pdbe-citations 1byo" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Plastocyanin 3D structures|Plastocyanin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Silene latifolia subsp. alba]] | [[Category: Silene latifolia subsp. alba]] | ||
[[Category: Gotowda M]] | |||
[[Category: Gotowda | [[Category: Hibino T]] | ||
[[Category: Hibino | [[Category: Inoue T]] | ||
[[Category: Inoue | [[Category: Kai Y]] | ||
[[Category: Kai | [[Category: Li C]] | ||
[[Category: Li | [[Category: Sugawara H]] | ||
[[Category: Sugawara | [[Category: Takabe T]] | ||
[[Category: Takabe | |||
Latest revision as of 08:46, 9 August 2023
WILD-TYPE PLASTOCYANIN FROM SILENEWILD-TYPE PLASTOCYANIN FROM SILENE
Structural highlights
FunctionPLAS_SILLB Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedPlastocyanin functions as an electron carrier between the cytochrome b6f complex and photosystem I. The crystal structures of the wild-type and E43K/D44K double mutant from the higher plant, Silene, have been determined at 2.0 and 1.75 A resolution, respectively. The wild-type plastocyanin comprises two monomers per asymmetric unit, one of which shows the unusually great distance between the copper ion and the Ndelta1 atom of H87 because of the hydrogen bond network formation between H87 and symmetry-related G10. The root mean square deviation for Ca atoms between the wild-type and mutant plastocyanins is 0.44 A, however, the electrostatic potential maps of their molecular surfaces are remarkably different. The low electron-transfer rate in the E43K/D44K mutant results from the hindrance of electrostatic interactions, not from the structural change due to the mutation. Crystal structures of wild-type and mutant plastocyanins from a higher plant, Silene.,Sugawara H, Inoue T, Li C, Gotowda M, Hibino T, Takabe T, Kai Y J Biochem. 1999 May;125(5):899-903. PMID:10220581[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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