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| [[Image:2dgc.png|left|200px|thumb|Crystal structure of Gcn4 complex with DNA [[2dgc]]]]
| | <StructureSection load='2dgc' size='350' side='right' caption='Structure of Gcn4 leucine zipper domain complex with DNA (PDB entry [[2dgc]])' scene=''54/540145/Biological_assembly/1''> |
| {{STRUCTURE_2dgc| PDB=2dgc | SIZE=400| SCENE= |right|CAPTION=Gcn4 complex with DNA [[2dgc]] }}
| | == Function == |
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| | '''Gcn4''' or '''General control protein Gcn4''' is a yeast transcriptional activator. It belongs to a large family of eukaryotic transcription factors including Fos, Jun and CREB. <ref>PMID:7934812</ref> Gcn4-pLI is a 33-residue peptide of the dimerization domain of Gcn4. See also [[Intrinsically Disordered Protein]]. |
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| | == Structural highlights == |
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| | Gcn4 and other family members have a DNA recognition motif consisting of a coiled-coil dimerization element, the leucine-zipper, and an adjoining basic region, which mediates DNA binding. This basic region is largely unstructured in the absence of DNA, addition of DNA containing a Gcn4 binding site induces the transition of this region from unstructured to α-helical. |
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| | | See [[Coiled coil]]. |
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| '''Gcn4''' is a yeast transcriptional activator. It belongs to a large family of eukaryotic transcription factors including Fos, Jun and CREB. All family members have a DNA recognition motif consists of a coiled-coil dimerization element, the leucine-zipper, and an adjoining basic region, which mediates DNA binding. This basic region is largely unstructured in the absence of DNA, addition of DNA containing a Gcn4 binding site induce the transition of this region from unstructured to α-helical. See also [[Intrinsically Disordered Protein]].
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| == 3D Structures of Gcn4 == | | == 3D Structures of Gcn4 == |
| | [[Gcn4 3D Structures]] |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | </StructureSection> |
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| [[2zta]], [[1w5i]], [[2b1f]], [[2b22]], [[2ag3]], [[2oxj]], [[2oxk]], [[2r2v]], [[4c46]] – yGcn4 - yeast <br />
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| [[2ovn]], [[2l56]] – yGcn4 - NMR<br />
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| [[1u2u]] – yGcn4 dimer - NMR<br />
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| [[4dmd]], [[1kd8]], [[1kd9]], [[1kdd]], [[4hu5]], [[4hu6]] – yGcn4 dimer <br />
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| [[4dme]] – yGcn4 trimer <br />
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| [[3ck4]], [[3crp]], [[2ipz]] – yGcn4 tetramer <br />
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| [[1gcl]], [[1swi]], [[1ce9]], [[1w5g]], [[1w5h]], [[1w5j]], [[1w5k]], [[1w5l]], [[1unt]], [[1unu]], [[1unv]], [[1unw]], [[1unx]], [[1uny]], [[1unz]], [[1uo0]], [[1uo1]], [[1uo2]], [[1uo3]], [[1uo4]], [[1uo5]], [[1u9f]], [[1u9g]], [[1u9h]], [[2bni]], [[2cce]], [[2ccf]], [[2ccn]], [[2ahp]], [[2hy6]], [[3i1g]], [[2wpy]], [[2wpz]], [[2wq0]], [[2wq1]], [[2wq2]], [[2wq3]], [[3k7z]], [[3gjp]], [[3m48]] – yGcn4 (mutant)<br />
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| [[1zii]], [[1zik]], [[1zil]] – yGcn4 dimer (mutant)<br />
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| [[2o7h]], [[1gcm]], [[1zij]], [[1zim]], [[1piq]], [[1ij0]], [[1ij1]], [[1ij2]], [[1ij3]], [[1rb4]], [[1rb5]], [[1rb6]] – yGcn4 trimer (mutant)<br />
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| [[2nrn]] – yGcn4 tetramer (mutant)<br />
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| [[1fmh]] – yGcn4 dimer (mutant) - NMR<br />
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| [[1c94]] – yGcn4 retro<br />
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| [[1ysa]], [[1dgc]], [[2dgc]] – yGcn4 dimer + DNA <br />
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| [[2lpb]] – yGcn4 + mediator of RNA polymerase II<br />
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| ===Gcn4 fusion proteins===
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| [[1gk6]] – yGcn4/vimentin<br />
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| [[2wg5]] – yGcn4/proteasome-activating nucleotidase<br />
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| [[2wg6]] – yGcn4 (mutant)/proteasome-activating nucleotidase<br />
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| [[2yny]], [[2ynz]], [[2yo0]], [[2yo1]], [[2yo2]], [[2yo3]], [[3zmf]] – yGcn4/inner membrane protein<br />
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| [[3i5c]] – yGcn4/WSPR response regulator<br />
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| [[3p8m]] – yGcn4/dynein<br />
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| [[4g2k]], [[1env]], [[1fav]], [[1gcl]], [[2q5u]], [[2q7c]], [[3g9r]] – yGcn4/virus envelope glycoprotein<br />
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| [[1kql]], [[2z5i]] – yGcn4/α-tropomyosin<br />
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| [[2z5h]] – yGcn4/α-tropomyosin + troponin T<br />
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| [[3bas]], [[3bat]] – yGcn4/myosin<br />
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| [[1wvl]], [[2d3e]], [[2efr]], [[2efs]], [[2g9j]] – yGcn4/SAC7D + DNA<br
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| [[4m8m]] – yGcn4/plexin<br />/>
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| [[1ld4]] – yGcn4 + virus coat protein + virus spike glycoprotein<br />
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| | == References == |
| | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |