Malate synthase: Difference between revisions

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<StructureSection load='' size='350' side='right' caption='Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry [[2gq3]])' scene='57/573146/Cv/1'>
<StructureSection load='' size='350' side='right' caption='Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry [[2gq3]])' scene='57/573146/Cv/1'>
== Function ==     
== Function ==     
'''Malate synthase''' (MS) catalyzes the reversible conversion of acetyl-CoA, glyoxylate and water to [[R/S nomencalture|(S)]]-malate and CoA.  MS participates in pyruvate, glyoxylate and dicarboxylate metabolism.  There are 2 isozymes of MS.  '''MS G''' is formed during growth on glycolate<ref>PMID:12930982</ref> and '''MS A''' which metabolizes glyoxylate formed in the dissimilation of acetate.
'''Malate synthase''' (MS) catalyzes the reversible conversion of acetyl-CoA, glyoxylate and water to [[R/S nomenclature|(S)]]-malate and CoA.  MS participates in pyruvate, glyoxylate and dicarboxylate metabolism.  There are 2 isozymes of MS.  '''MS G''' is formed during growth on glycolate<ref>PMID:12930982</ref> and '''MS A''' which metabolizes glyoxylate formed in the dissimilation of acetate.
 
See also: [[Glyoxylate cycle]]


== Structural highlights ==
== Structural highlights ==
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<scene name='57/573146/Cv/10'>Acetyl-CoA binding site</scene>.
<scene name='57/573146/Cv/10'>Acetyl-CoA binding site</scene>.
</StructureSection>


==3d structures of malate synthase==
==3d structures of malate synthase==
[[Malate synthase 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
{{#tree:id=OrganizedByTopic|openlevels=0|
 
*'''Malate synthase'''
 
**[[1y8b]], [[2jqx]] – EcMS G (mutant) – ''Escherichia coli'' - NMR<br />
**[[3cuz]], [[3cv1]] – EcMS A<br />
**[[4ex4]] – MS G + Mg – ''Mycobacterium leprae''<br />
**[[5h8p]] – MtMS G  – ''Mycobacterium tuberculosis''<br />
**[[5h8m]] – MtMS G (mutant) + Mg <br />
**[[3cux]] – MS + Mg – ''Bacillus anthracis''<br />
**[[5oas]] – PaMS G + Mg – ''Pseudomonas aeruginosa''<br />
 
*Malate synthase binary complex
 
**[[1d8c]] – EcMS G (mutant) + glyoxylate + Mg <br />
**[[3cv2]] – EcMS A + oxalate + Mg <br />
**[[1n8i]] – MtMS G + glyoxylate + Mg <br />
**[[5h8u]] – MtMS G + malate + Mg <br />
**[[56axe]] – MtMS G + acetyl CoA + Mg <br />
**[[5c7v]], [[5cah]], [[5cjm]], [[5e9x]] – MtMS G (mutant) + pyrrole derivative + Mg <br />
**[[5cew]] – MtMS G (mutant) + pyridine derivative + Mg <br />
**[[5cjn]] – MtMS G (mutant) + quinoxaline derivative + Mg <br />
**[[5dx7]] – MtMS G (mutant) + isoxazole derivative + Mg <br />
**[[5c9r]], [[5c9u]], [[5c9w]], [[5cbj]], [[5ccz]] – MtMS G (mutant) + phenyl derivative + Mg <br />
**[[5c9x]], [[5cbi]] – MtMS G (mutant) + benzoate derivative + Mg <br />
**[[5cak]], [[5cbb]], [[5cc3]], [[5cc5]], [[5cc6]], [[5cc7]], [[5drc]], [[5dri]], [[5ecv]] – MtMS G (mutant) + indole derivative + Mg <br />
**[[5t8g]] – MtMS G (mutant) + thiophene derivative + Mg <br />
**[[3s9i]], [[3s9z]], [[3sad]], [[3saz]], [[3sb0]] – MtMS G (mutant) + inhibitor + Mg <br />
**[[3oyx]], [[3pug]] – HvMS G + glyoxylate + Mg – ''Haloferax volcanii''<br />
**[[5tao]] – HvMS G + glyoxylate + Pb<br />
**[[5vfb]] – PaMS + glycolate + Ni<br />
 
*Malate synthase ternary complex


**[[1p7t]] – EcMS G (mutant) + pyruvate + Mg + acetyl-CoA<br />
**[[3oyz]] – HvMS + pyruvate + Mg + acetyl-CoA<br />
**[[1n8w]] – MtMS G + glyoxylate + Mg + CoA<br />
**[[2gq3]] – MtMS G + malate + Mg + CoA<br />
}}
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 12:03, 11 January 2023

Function

Malate synthase (MS) catalyzes the reversible conversion of acetyl-CoA, glyoxylate and water to (S)-malate and CoA. MS participates in pyruvate, glyoxylate and dicarboxylate metabolism. There are 2 isozymes of MS. MS G is formed during growth on glycolate[1] and MS A which metabolizes glyoxylate formed in the dissimilation of acetate.

See also: Glyoxylate cycle

Structural highlights

. The ternary complex contains malate, acetyl-CoA and Mg+2 ion[2].

. Water molecules are shown as red spheres.

.

3d structures of malate synthase

Malate synthase 3D structures


Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry 2gq3)

Drag the structure with the mouse to rotate

ReferencesReferences

  1. ↑ Anstrom DM, Kallio K, Remington SJ. Structure of the Escherichia coli malate synthase G:pyruvate:acetyl-coenzyme A abortive ternary complex at 1.95 A resolution. Protein Sci. 2003 Sep;12(9):1822-32. PMID:12930982
  2. ↑ Anstrom DM, Remington SJ. The product complex of M. tuberculosis malate synthase revisited. Protein Sci. 2006 Aug;15(8):2002-7. PMID:16877713 doi:15/8/2002

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