Malate synthase: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman, Karsten Theis

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-<StructureSection load='2gq3' size='350' side='right' caption='Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry [[2gq3]])' scene=''>
+<StructureSection load='' size='350' side='right' caption='Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry [[2gq3]])' scene='57/573146/Cv/1'>
 == Function ==
-'''Malate synthase''' (MS) catalyzes the reversible conversion of acetyl-CoA, glyoxylate and water to (S)-malate and CoA.  MS participates in pyruvate, glyoxylate and dicarboxylate metabolism.  There are 2 isozymes of MS.  '''MS G''' is formed during growth on glycolate<ref>PMID:12930982</ref> and '''MS A''' which metabolizes glyoxylate formed in the dissimilation of acetate.
+'''Malate synthase''' (MS) catalyzes the reversible conversion of acetyl-CoA, glyoxylate and water to [[R/S nomenclature|(S)]]-malate and CoA.  MS participates in pyruvate, glyoxylate and dicarboxylate metabolism.  There are 2 isozymes of MS.  '''MS G''' is formed during growth on glycolate<ref>PMID:12930982</ref> and '''MS A''' which metabolizes glyoxylate formed in the dissimilation of acetate.
+See also: [[Glyoxylate cycle]]
 == Structural highlights ==
-MS active site pocket is situated between the TIM barrle and the C-terminal.  The ternary complex contains malate, acetylCoA and Mg+2 ion<ref>PMID:16877713</ref>.
+<scene name='57/573146/Cv/8'>MS active site pocket is situated between the TIM barrel and the C-terminal</scene>.  The ternary complex contains malate, acetyl-CoA and Mg+2 ion<ref>PMID:16877713</ref>.
-</StructureSection>
-==3d structures of malate synthase==
+<scene name='57/573146/Cv/9'>Malate/Mg+2 ion binding site</scene>. Water molecules  are shown as red spheres.
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+<scene name='57/573146/Cv/10'>Acetyl-CoA binding site</scene>.
-{{#tree:id=OrganizedByTopic|openlevels=0|
-*'''Malate synthase'''
+==3d structures of malate synthase==
+[[Malate synthase 3D structures]]
-**[[1y8b]], [[2jqx]] – EcMS G (mutant) – ''Escherichia coli'' - NMR<br />
+</StructureSection>
-**[[3cuz]], [[3cv1]] – EcMS A<br />
-**[[4ex4]] – MS G + Mg – ''Mycobacterium leprae''<br />
-**[[3cux]] – MS + Mg – ''Bacillus anthracis''<br />
-*Malate synthase binary complex
-**[[1d8c]] – EcMS G (mutant) + glyoxylate + Mg <br />
-**[[3cv2]] – EcMS A + oxalate + Mg <br />
-**[[1n8i]] – MtMS G + glyoxylate + Mg – ''Mycobacterium tuberculosis''<br />
-**[[3oyx]], [[3pug]] – HvMS + glyoxylate + Mg – ''Haloferax volcanii''<br />
-**[[3s9i]], [[3s9z]], [[3sad]], [[3saz]], [[3sb0]] – MtMS G (mutant) + inhibitor + Mg <br />
-*Malate synthase ternary complex
-**[[1p7t]] – EcMS G (mutant) + pyruvate + Mg + acetyl-CoA<br />
-**[[3oyz]] – HvMS + pyruvate + Mg + acetyl-CoA<br />
-**[[1n8w]] – MtMS G + glyoxylate + Mg + CoA<br />
-**[[2gq3]] – MtMS G + malate + Mg + CoA<br />
-}}
 == References ==
 <references/>
 [[Category:Topic Page]]