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| <StructureSection load='1vol' size='400' side='right' caption='Structure of human TFIIB (grey) complex with TATA-binding protein (green) and DNA (PDB entry [[1vol]])' scene=''> | | <StructureSection load='' size='350' side='right' caption='Structure of human TFIIB (cyan) complex with TATA-binding protein (green) and DNA (PDB entry [[1vol]])' scene='51/517377/Cv/1' pspeed='8'> |
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| '''Transcription initiation factors''' (TFII) are proteins which enable RNA polymerase II to bind to the DNA template in order to start the transcription process<ref>PMID:8870495</ref><ref>PMID:10022900</ref>.<br /> | | '''Transcription initiation factors''' (TFII) are proteins which enable RNA polymerase II to bind to the DNA template in order to start the transcription process<ref>PMID:8870495</ref><ref>PMID:10022900</ref>.<br /> |
| * '''TFIIA''' is one of several TFs which are required for transcription. TFIIA aids in binding the TATA-binding protein (TBP) to the TATA box containing sequence of the DNA.<br /> | | * '''TFIIA''' is one of several TFs which are required for transcription. TFIIA aids in binding the TATA-binding protein (TBP) to the TATA box containing sequence of the DNA.<br /> |
| * '''TFIIB''' bridges between TFIID and RNA polymerase II<ref>PMID:1876184</ref>.<br /> | | * '''TFIIB''' bridges between TFIID and RNA polymerase II<ref>PMID:1876184</ref>.<br /> |
| | * '''TFIIIB''' is the core RNA polymerase III initiation factor. It recruits pol III to the transcriptional start site<ref>PMID:17073756</ref>.<br /> |
| * '''TFIID''' recognizes the promoter sequence on the DNA.<br /> | | * '''TFIID''' recognizes the promoter sequence on the DNA.<br /> |
| * '''TFIIE''' both subunits are are essential to form a preinitiation complex.<ref>PMID:1956398</ref><br /> | | * '''TFIIE''' both subunits are are essential to form a preinitiation complex.<ref>PMID:1956398</ref><br /> |
| * '''TFIIF''' binds RNA polymerase III prohibiting it to bind to DNA outside the promoter. | | * '''TFIIF''' binds RNA polymerase III prohibiting it to bind to DNA outside the promoter.<br /> |
| </StructureSection> | | * '''TF RRN''' initiate transcription by RNA polymerase I in yeast.<ref>PMID:8670901</ref><br /> |
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| | <scene name='51/517377/Cv/3'>Human TFIIB complex with TATA-binding protein and DNA</scene> (PDB entry [[1vol]]). |
| ==3D structures of transcription initiation factors== | | ==3D structures of transcription initiation factors== |
| | [[Transcription initiation factors 3D structures]] |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | </StructureSection> |
| {{#tree:id=OrganizedByTopic|openlevels=0|
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| *TFIIA
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| **[[1ytf]], [[1nh2]], [[1rm1]] – yTFIIA + TBP + DNA – yeast<br />
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| **[[5fur]] – yTFIIA + yTFIIF + TATA-binding protein + DNA - CryoEM<br />
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| **[[1nvp]] – hTFIIA + TATA-binding protein + DNA – human<br />
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| *TFIIB
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| **[[1pft]] – TFIIB N terminal – ''Pyrococcus furiosus'' – NMR<BR />
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| **[[1tfb]] – hTFIIB - NMR<BR />
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| **[[1dl6]] - hTFIIB N terminal – NMR<br />
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| **[[1rly]] - hTFIIB Zn ribbon domain + Zn – NMR<br />
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| **[[1ro4]] - hTFIIB Zn ribbon domain – NMR<br />
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| **[[1ais]], [[1d3u]] - TFIIB C terminal + TBP + DNA – ''Pyrococcus woesei''
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| *TFIIIB
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| **[[4roc]], [[4rod]], [[4roe]] – hTFIIIB + TATA-binding protein + DNA <br />
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| *TFIID
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| **[[1bh8]] – hTFIID<BR />
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| **[[1eqf]] – hTFIID subunit 1<br />
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| **[[3uv5]] – hTFIID subunit 1 bromodomain 1-2<br />
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| **[[3uv4]] – hTFIID subunit 1 bromodomain 2<br />
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| **[[3aad]] – hTFIID subunit 1 bromodomain 1-2 + histone chaperone ASF1A<br />
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| **[[4yym]], [[4yyn]] – hTFIID subunit 1 bromodomain 2 + histone peptide<br />
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| **[[5c13]] – hTFIID subunit 3 + histone peptide<br />
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| **[[5i1q]], [[5i29]] – hTFIID subunit 1 bromodomain 2 + pyrrolopyridone<br />
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| **[[5eg2]] - hTFIID subunit 10 + SETD7 + SAH<br />
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| **[[4wv4]] - hTFIID subunits 8+10 <br />
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| **[[4grw]] - hTFIID subunits 1+7 <br />
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| **[[4oy2]] - yTFIID subunits 1+7 <br />
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| **[[5iok]], [[5o7e]] – yTFIID subunit 14 + histone peptide<br />
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| **[[1tba]] – TFIID – ''Drosophila melanogaster'' - NMR<BR />
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| **[[1bh9]] – hTFIID + PCMBS<BR />
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| **[[1qn3]], [[1qn4]], [[1qn5]], [[1qn6]], [[1qn7]], [[1qn8]], [[1qn9]], [[1qna]], [[1qnb]], [[1qnc]], [[1qne]] – TFIID + DNA – ''Arabidopsis thaliana''
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| *TFIIE
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| **[[1vd4]] - hTFIIB Zn finger domain – NMR<br />
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| **[[2rnq]] – hTFIIE C terminal - NMR<br />
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| **[[2rnr]] – hTFIIE C terminal + TFIIH PH domain- NMR<br />
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| *TFIIF
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| **[[1f3u]] – hTFIIF<br />
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| **[[1nha]] – hTFIIF C terminal - NMR<br />
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| **[[1i27]] – hTFIIF C terminal (mutant)<br />
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| **[[1j2x]] – hTFIIF C terminal + RNA polymerase CTD phosphatase peptide<br />
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| **[[1onv]] – hTFIIF C terminal + serine phosphatase peptide - NMR<br />
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| **[[2k7l]] – hTFIIF C terminal + FCP1 peptide - NMR<br />
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| *TF in RNA polymerase II
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| **[[5fyw]] – yTFIIA + yTFIIE + yTFIIF + DNA <br />
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| **[[5fz5]] – yTFIIA + yTFIIE + yTFIIF + DNA – Cryo EM<br />
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| **[[4v1o]], [[4v1n]] – yTFIIB + yTFIIF – Cryo EM<br />
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| **[[3k1f]], [[3k7a]], [[4bbr]], [[4bbs]] – yTFIIB <br />
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| **[[5iy6]], [[5iy7]], [[5iy8]], [[5iy9]], [[5iya]], [[5iyb]], [[5iyc]], [[5iyd]] – hTFIIA + hTFIIB + hTFIIE + hTFIIF + hTFIIH + DNA – Cryo EM<br />
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| **[[5fmf]] – yTFIIA + yTFIIB + yTFIIE + yTFIIF + yTFIIH + DNA – Cryo EM<br />
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| *TF RRN3
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| **[[5g5l]] – yTF RRN3 in RNA polymerase I<br />
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| }}
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| == References == | | == References == |
| <references/> | | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |