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| {{STRUCTURE_3uw0| PDB=3uw0 | SIZE=400| SCENE= |right|CAPTION=Pectin methylesterase [[3uw0]] }}
| | <StructureSection load='3c5w' size='350' side='right' caption='Human protein phosphatase methylesterase (green) complex with protein phosphatase 2A subunit A (cyan) and subunit C (magenta) (PDB entry [[3c5w]])' scene='48/489289/Cv/1'> |
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| '''Methylesterase''' (ME) removes a methyl group from the Υ-glutamyl methyl esther residues of methyl-accepting chemotaxis proteins. ME participates in several metabolic pathways. Protein phosphatase ME is involved in the reversible methylation of protein phosphatase 2A which is active in cellular regulation.<br />
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| * '''CheB ME''' is a phosphorylation-activated response regulator involved in reversible modification of bacterial chemotaxis receptors.<br />
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| * '''Pectin ME''' causes demethylation of cell wall polygalactorunanas. It is involved in pectin digestion. The reaction products liberate frothing agents causing bloating in cattle.<br />
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| * '''Aclacinomycin ME''' modifies the aklavinone skeleton in the biosynthesis of anthracyclins in ''Streptomyces'' species. <br />
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| * '''Protein phosphatase ME''' is a key regulator of protein phosphatase 2A.<br />
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| * '''4-o-methyl-glucuronoyl ME''' has a significant role in biomass degradation.<br />
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| ==3D structures of methylesterase== | | __TOC__ |
| | == Function == |
| | '''Methylesterase''' (ME) removes a methyl group from the Υ-glutamyl methyl esther residues of methyl-accepting chemotaxis proteins. ME participates in several metabolic pathways. <br /> |
| | * '''CheB ME''' is a phosphorylation-activated response regulator involved in reversible modification of bacterial chemotaxis receptors<ref>PMID:2991277</ref>. See [[Chemotaxis protein]].<br /> |
| | * '''Pectin ME''' causes demethylation of cell wall polygalactorunanas. It is involved in pectin digestion. The reaction products liberate frothing agents causing bloating in cattle<ref>PMID:11544130</ref>.<br /> |
| | * '''Aclacinomycin ME''' modifies the aklavinone skeleton in the biosynthesis of anthracyclins in ''Streptomyces'' species<ref>PMID:11934504</ref>. <br /> |
| | * '''Protein phosphatase ME''' is involved in the reversible methylation of protein phosphatase 2A which is active in cellular regulation<ref>PMID:24928782</ref>.<br /> |
| | * '''4-o-methyl-glucuronoyl ME''' has a significant role in biomass degradation<ref>PMID:16876163</ref>.<br /> |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | == Structural highlights == |
| | *<scene name='48/489289/Cv/6'>Human protein phosphatase methylesterase with protein phosphatase 2A subunits A and C</scene>. |
| | *<scene name='48/489289/Cv/5'>Protein phosphatase ME binds to the active site of subunit C of phospholipase 2A</scene>. It inactivates phospholipase 2A by interacting with the latter's subunit C by removing two catalytic Mn+2 ions from it<ref>PMID:18394995</ref>. |
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| ===CheB methylesterase=== | | ==3D structures of methylesterase== |
| | | [[Methylesterase 3D structures]] |
| [[1chd]], [[1a2o]] – ME – ''Salmonella typhimurium''<br />
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| [[3sft]] – TmME catalytic domain – ''Thermotoga maritima''<br />
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| [[3t8y]] – TmME response regulator domain
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| ===Pectin methylesterase===
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| [[1qjv]] – EcME – ''Erwinia chrysanthemi''<br /> | |
| [[1gq8]] – ME – carrot<br />
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| [[3uw0]] – ME – ''Yersinia enterocolitica''<br />
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| [[1xg2]] – ME + protein inhibitor – ''Solanum lycopersicum''<br />
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| [[2nsp]], [[2nst]], [[2nt6]], [[2nt9]] – EcME (mutant) + hexasaccharide<br />
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| [[2ntp]], [[2ntb]], [[2ntq]] – EcME + hexasaccharide
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| ===Aclacinomycin methylesterase===
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| [[1q0r]], [[1q0z]] – ME + product analog – ''Streptomyces purpurascens''
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| ===Protein phosphatase methylesterase===
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| [[3c5v]] – hME1 – human<br />
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| [[3c5w]] – hME1 + protein phosphatase 2A
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| ===4-o-methyl-glucuronoyl methylesterase===
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| [[4g4g]] – MtME – ''Myceliophthora thermophila''<br />
| | </StructureSection> |
| [[4g4i]] – MtME (mutant)<br />
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| [[4g4j]] – MtME (mutant) + 4-o-methyl-β-D-glucopyranuronate<br />
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| | == References == |
| | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |