Proteopedia

Methylesterase


Function

Methylesterase (ME) removes a methyl group from the Υ-glutamyl methyl esther residues of methyl-accepting chemotaxis proteins. ME participates in several metabolic pathways.

  • CheB ME is a phosphorylation-activated response regulator involved in reversible modification of bacterial chemotaxis receptors[1]. See Chemotaxis protein.
  • Pectin ME causes demethylation of cell wall polygalactorunanas. It is involved in pectin digestion. The reaction products liberate frothing agents causing bloating in cattle[2].
  • Aclacinomycin ME modifies the aklavinone skeleton in the biosynthesis of anthracyclins in Streptomyces species[3].
  • Protein phosphatase ME is involved in the reversible methylation of protein phosphatase 2A which is active in cellular regulation[4].
  • 4-o-methyl-glucuronoyl ME has a significant role in biomass degradation[5].

Structural highlights

  • .
  • . It inactivates phospholipase 2A by interacting with the latter's subunit C by removing two catalytic Mn+2 ions from it[6].

3D structures of methylesterase

Methylesterase 3D structures


Human protein phosphatase methylesterase (green) complex with protein phosphatase 2A subunit A (cyan) and subunit C (magenta) (PDB entry 3c5w)

Drag the structure with the mouse to rotate

ReferencesReferences

  1. ↑ Simms SA, Keane MG, Stock J. Multiple forms of the CheB methylesterase in bacterial chemosensing. J Biol Chem. 1985 Aug 25;260(18):10161-8. PMID:2991277
  2. ↑ Micheli F. Pectin methylesterases: cell wall enzymes with important roles in plant physiology. Trends Plant Sci. 2001 Sep;6(9):414-9. PMID:11544130
  3. ↑ Wang Y, Niemi J, Mantsala P. Modification of aklavinone and aclacinomycins in vitro and in vivo by rhodomycin biosynthesis gene products. FEMS Microbiol Lett. 2002 Feb 19;208(1):117-22. PMID:11934504
  4. ↑ Wandzioch E, Pusey M, Werda A, Bail S, Bhaskar A, Nestor M, Yang JJ, Rice LM. PME-1 modulates protein phosphatase 2A activity to promote the malignant phenotype of endometrial cancer cells. Cancer Res. 2014 Aug 15;74(16):4295-305. doi: 10.1158/0008-5472.CAN-13-3130. Epub, 2014 Jun 13. PMID:24928782 doi:http://dx.doi.org/10.1158/0008-5472.CAN-13-3130
  5. ↑ Spanikova S, Biely P. Glucuronoyl esterase--novel carbohydrate esterase produced by Schizophyllum commune. FEBS Lett. 2006 Aug 21;580(19):4597-601. Epub 2006 Jul 21. PMID:16876163 doi:http://dx.doi.org/10.1016/j.febslet.2006.07.033
  6. ↑ Xing Y, Li Z, Chen Y, Stock JB, Jeffrey PD, Shi Y. Structural mechanism of demethylation and inactivation of protein phosphatase 2A. Cell. 2008 Apr 4;133(1):154-63. PMID:18394995 doi:http://dx.doi.org/10.1016/j.cell.2008.02.041

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Michal Harel, Alexander Berchansky
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