Dethiobiotin synthetase: Difference between revisions

Latest revision as of 12:24, 5 June 2019

Dethiobiotin synthetase (DTBS) or ATP-depenent dethiobiotin synthetase catalyzes the first ring closure in the biotin synthesis. The reaction transforms 7,8-diaminononanoate (DAN), ATP and CO2 to dethiobiotin (DTB), ADP and phosphate.^[1]

Structural highlights

The .^[2]

3D dethiobiotin synthetase

Dethiobiotin synthetase 3D structures

Dethiobiotin synthetase complex with dethiobiotin, ATP and Mg+2 ion (green) (PDB entry 1a82)

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ReferencesReferences

↑ Krell K, Eisenberg MA. The purification and properties of dethiobiotin synthetase. J Biol Chem. 1970 Dec 25;245(24):6558-66. PMID:4921568
↑ Kack H, Gibson KJ, Lindqvist Y, Schneider G. Snapshot of a phosphorylated substrate intermediate by kinetic crystallography. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5495-500. PMID:9576910

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

[1] Krell K, Eisenberg MA. The purification and properties of dethiobiotin synthetase. J Biol Chem. 1970 Dec 25;245(24):6558-66. PMID:4921568

[2] Kack H, Gibson KJ, Lindqvist Y, Schneider G. Snapshot of a phosphorylated substrate intermediate by kinetic crystallography. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5495-500. PMID:9576910

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- {{STRUCTURE_1bs1|  PDB=1bs1  | SIZE=400| SCENE= |right|   CAPTION=Dethiobiotin synthetase complex with dethiobiotin, ADP and Mg+2 ion, [[1bs1]] }}
+<StructureSection load='1a82' size='450' side='right' caption='Dethiobiotin synthetase complex with dethiobiotin, ATP and Mg+2 ion (green) (PDB entry [[1a82]])' scene='49/493028/Cv/1'>
-<!--
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-'''Dethiobiotin synthetase''' (DTBS) catalyzes the first ring closure in the biotin synthesis.  The reaction transforms 7,8-diaminononanoate (DAN), ATP and CO2 to dethiobiotin (DTB), ADP and phosphate.
-==3D dethiobiotin synthetase==
+'''Dethiobiotin synthetase''' (DTBS) or '''ATP-depenent dethiobiotin synthetase''' catalyzes the first ring closure in the biotin synthesis.  The reaction transforms 7,8-diaminononanoate (DAN), ATP and CO2 to dethiobiotin (DTB), ADP and phosphate.<ref>PMID:4921568</ref>
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+== Structural highlights ==
-===DTBS===
+The <scene name='49/493028/Cv/5'>active site contains the substrate (DAN), Mg+2 ion and ATP</scene>.<ref>PMID:9576910</ref>
-[[1dbs]], [[1dts]], [[1byi]] – EcDTBS – ''Escherichia coli''<br />
+==3D dethiobiotin synthetase==
-[[2qmo]] – HpDTBS – ''Helicobacter pylori''<br />
+[[Dethiobiotin synthetase 3D structures]]
-[[3fgn]] – MtDTBS – ''Mycobacterium tuberculosis''<br />
-[[3of5]] – DTBS – ''Francicella tularensis''
-===DTBS binary complex===
-[[3fmf]] – MtDTBS + DAN<br />
-[[1dad]] – EcDTBS + ADP<BR />
-[[1dae]] - EcDTBS + nonanedioate derivative<br />
-[[3fmi]] – MtDTBS + aminopelargonic acid<br />
-[[1dai]] - EcDTBS + DAN
-===DTBS ternary complex===
-[[3qxc]] - HpDTBS + Mg + ATP<BR />
-[[1daf]] - EcDTBS + DAN + ADP<BR />
-[[1dag]] - EcDTBS + DAN + ATP derivative<br />
-[[3qxj]] - HpDTBS + Mg + ATP<BR />
-[[3qxs]] - HpDTBS + Mg + phosphoaminophosphonic acid
-===DTBS quaternary complex===
-[[1dah]] - EcDTBS + DAN + Mn + ATP derivative<br />
-[[1bs1]] - EcDTBS + DTB + Mg + ADP<BR />
-[[1a82]] - EcDTBS + DAN + Mg + ATP<BR />
-[[3fpa]] - MtDTBS + DTB + Mg + phosphate <br />
-[[3mle]] - HpDTBS + Mg + phosphate + ADP<BR />
-[[3qy0]] - HpDTBS + Mg + phosphate + GDP<BR />
-===DTBS higher complexes===
-[[1dam]] - EcDTBS + DTB + Mg + phosphate + ADP<BR />
+</StructureSection>
-[[1dak]] - EcDTBS + DAN + mixed anhydride + Mg + phosphate + ADP<BR />
-[[3qxh]] - HpDTBS + aminooctanoic acid + Mg + phosphate + ADP<BR />
-[[3qxx]] - HpDTBS + aminooctanoic acid + Mg + phosphate + GTP<BR />
+== References ==
+<references/>
 [[Category:Topic Page]]