SelB: Difference between revisions
New page: {{STRUCTURE_1wsu | PDB=1wsu | SCENE=Goodsell_Sandbox/1wsu-recognition0/1 }} Molecular Recognition in SelB Elongation factor SelB uses several methods to recognize the selenocysteine i... |
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<StructureSection load='1wsu' size='350' side='right' scene=''' caption='Elongation factor SelB C terminal tetramer complex with RNA [[1wsu]]'> | |||
'''Elongation factor SelB''' uses several methods to recognize the selenocysteine insertion sequence found in messenger RNA. The insertion sequence forms a hairpin loop with an unstacked guanine base in the loop. This guanine forms | |||
<scene name='SelB_Recognition/1wsu-recognition1/3'>specific hydrogen bonds with the protein and with two bridging water molecules (shown in cyan in the jmol).</scene> | |||
Elongation factor SelB uses several methods to recognize the selenocysteine insertion sequence found in messenger RNA. The insertion sequence forms a hairpin loop with an unstacked guanine base in the loop. This guanine forms | |||
<scene name=' | |||
The planar base is also sandwiched between | The planar base is also sandwiched between | ||
<scene name='Sandbox_14/1wsu-recognition2/2'>three amino acids</scene>in the protein, which form a tight hydrophobic pocket. This is best seen using a | <scene name='Sandbox_14/1wsu-recognition2/2'>three amino acids</scene> in the protein, which form a tight hydrophobic pocket. This is best seen using a | ||
<scene name='Sandbox_14/1wsu-recognition3/1'>spacefilling diagram.</scene> | <scene name='Sandbox_14/1wsu-recognition3/1'>spacefilling diagram.</scene> | ||
The overall shape of the hairpin is recognized by contacts with several | The overall shape of the hairpin is recognized by contacts with several | ||
<scene name=' | <scene name='SelB_Recognition/1wsu-recognition4/1'>arginine and lysine amino acids.</scene> | ||
In particular, notice the intimate contact formed by both the charged nitrogen and hydrophobic carbon chain of | In particular, notice the intimate contact formed by both the charged nitrogen and hydrophobic carbon chain of | ||
<scene name='Sandbox_14/1wsu-recognition5/1'>arginine 606.</scene> | <scene name='Sandbox_14/1wsu-recognition5/1'>arginine 606.</scene> | ||
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This page complements the article on [http://www.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb104_1.html Selenocysteine Synthase] in the | |||
[http://www.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] Series. See also [[Teaching Scenes, Tutorials, and Educators' Pages]]. | |||
{{Clear}} | |||
</StructureSection> | |||
==3D structures of SelB== | |||
[[1lva]] – MtSelB C terminal – ''Moorella thermoacetica''<br /> | |||
[[2v9v]] - MtSelB winged-helix domains<br /> | |||
[[4aca]] – MmSelB – ''Methanococcus maripaludis'' | |||
===SelB complex with nucleotides=== | |||
[[4ac9]] – MmSelB + GDP <br /> | |||
[[4acb]] – MmSelB + GPPNHP<br /> | |||
[[1wsu]], [[2uwm]] - MtSelB C terminal + RNA<br /> | |||
[[2pjp]] – EcSelB mRNA-binding domain + RNA – ''Eschrichia coli''<br /> | |||
[[2ply]] - EcSelB mRNA-binding domain (mutant) + RNA | |||
==Additional Resources== | |||
For additional information, see: [[Translation]] | |||
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[[Category:Topic Page]] | |||