View source for 7vsq


==Crystal strcuture of the tandem B-Box domains of CONSTANS==
<StructureSection load='7vsq' size='340' side='right'caption='[[7vsq]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7vsq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VSQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VSQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vsq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vsq OCA], [https://pdbe.org/7vsq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vsq RCSB], [https://www.ebi.ac.uk/pdbsum/7vsq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vsq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CONS_ARATH CONS_ARATH] Transcription factor that acts in the long day flowering pathway and may mediate between the circadian clock and the control of flowering. Plays a role in the regulation of flowering time by acting on 'SUPPRESSOR OF OVEREXPRESSION OF CO1', 'TERMINAL FLOWER 1' and 'FLOWERING LOCUS T'. Also regulates P5CS2 and ACS10 (involved in proline and ethylene biosynthesis, respectively). Regulates the expression of NAKR1 by binding to the 5'-TGTG(N2-3)ATG-3' motif (PubMed:27255839).<ref>PMID:10834834</ref> <ref>PMID:11323677</ref> <ref>PMID:21950734</ref> <ref>PMID:27255839</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The transcription factor CONSTANS (CO) integrates day-length information to induce the expression of florigen FLOWERING LOCUS T (FT) in Arabidopsis. We recently reported that the C-terminal CCT domain of CO forms a complex with NUCLEAR FACTOR-YB/YC to recognize multiple cis-elements in the FT promoter, and the N-terminal tandem B-box domains form a homomultimeric assembly. However, the mechanism and biological function of CO multimerization remained unclear. Here, we report that CO takes on a head-to-tail oligomeric configuration via its B-boxes to mediate FT activation in long days. The crystal structure of B-boxes(CO) reveals a closely connected tandem B-box fold forming a continuous head-to-tail assembly through unique CDHH zinc fingers. Mutating the key residues involved in CO oligomerization resulted in a non-functional CO, as evidenced by the inability to rescue co mutants. By contrast, a transgene encoding a human p53-derived tetrameric peptide in place of the B-boxes(CO) rescued co mutant, emphasizing the essential role of B-boxes(CO) -mediated oligomerization. Furthermore, we found that the four TGTG-bearing cis-elements in FT proximal promoter are required for FT activation in long days. Our results suggest that CO forms a multimer to bind to the four TGTG motifs in the FT promoter to mediate FT activation.

Molecular basis of CONSTANS oligomerization in FLOWERING LOCUS T activation.,Zeng X, Lv X, Liu R, He H, Liang S, Chen L, Zhang F, Chen L, He Y, Du J J Integr Plant Biol. 2022 Jan 12. doi: 10.1111/jipb.13223. PMID:35023269<ref>PMID:35023269</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7vsq" style="background-color:#fffaf0;"></div>

==See Also==
*[[Constans|Constans]]
*[[3D structures of constans|3D structures of constans]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Du J]]
[[Category: Liu R]]
[[Category: Lv X]]