7vsq

Crystal strcuture of the tandem B-Box domains of CONSTANSCrystal strcuture of the tandem B-Box domains of CONSTANS

Structural highlights 7vsq is a 3 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.7Å Ligands: Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CONS_ARATH Transcription factor that acts in the long day flowering pathway and may mediate between the circadian clock and the control of flowering. Plays a role in the regulation of flowering time by acting on 'SUPPRESSOR OF OVEREXPRESSION OF CO1', 'TERMINAL FLOWER 1' and 'FLOWERING LOCUS T'. Also regulates P5CS2 and ACS10 (involved in proline and ethylene biosynthesis, respectively). Regulates the expression of NAKR1 by binding to the 5'-TGTG(N2-3)ATG-3' motif (PubMed:27255839). Publication Abstract from PubMed The transcription factor CONSTANS (CO) integrates day-length information to induce the expression of florigen FLOWERING LOCUS T (FT) in Arabidopsis. We recently reported that the C-terminal CCT domain of CO forms a complex with NUCLEAR FACTOR-YB/YC to recognize multiple cis-elements in the FT promoter, and the N-terminal tandem B-box domains form a homomultimeric assembly. However, the mechanism and biological function of CO multimerization remained unclear. Here, we report that CO takes on a head-to-tail oligomeric configuration via its B-boxes to mediate FT activation in long days. The crystal structure of B-boxes(CO) reveals a closely connected tandem B-box fold forming a continuous head-to-tail assembly through unique CDHH zinc fingers. Mutating the key residues involved in CO oligomerization resulted in a non-functional CO, as evidenced by the inability to rescue co mutants. By contrast, a transgene encoding a human p53-derived tetrameric peptide in place of the B-boxes(CO) rescued co mutant, emphasizing the essential role of B-boxes(CO) -mediated oligomerization. Furthermore, we found that the four TGTG-bearing cis-elements in FT proximal promoter are required for FT activation in long days. Our results suggest that CO forms a multimer to bind to the four TGTG motifs in the FT promoter to mediate FT activation. Molecular basis of CONSTANS oligomerization in FLOWERING LOCUS T activation.,Zeng X, Lv X, Liu R, He H, Liang S, Chen L, Zhang F, Chen L, He Y, Du J J Integr Plant Biol. 2022 Jan 12. doi: 10.1111/jipb.13223. PMID:35023269 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Constans 3D structures of constans

spinqualitylabelsall models 7vsq, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image