View source for 6fqb


==MurT/GatD peptidoglycan amidotransferase complex from Streptococcus pneumoniae R6==
<StructureSection load='6fqb' size='340' side='right'caption='[[6fqb]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6fqb]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FQB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FQB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fqb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fqb OCA], [https://pdbe.org/6fqb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fqb RCSB], [https://www.ebi.ac.uk/pdbsum/6fqb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fqb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MURT_STRR6 MURT_STRR6] The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide (PubMed:24044435, PubMed:30093673). The MurT subunit catalyzes the ATP-dependent amidation of D-glutamate residue of lipid II, converting it to an isoglutamine residue (PubMed:30093673).<ref>PMID:24044435</ref> <ref>PMID:30093673</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The universality of peptidoglycan in bacteria underlies the broad spectrum of many successful antibiotics. However, in our times of widespread resistance, the diversity of peptidoglycan modifications offers a variety of new antibacterials targets. In some Gram-positive species such as Streptococcus pneumoniae, Staphylococcus aureus, or Mycobacterium tuberculosis, the second residue of the peptidoglycan precursor, D-glutamate, is amidated into iso-D-glutamine by the essential amidotransferase MurT/GatD complex. Here, we present the structure of this complex at 3.0 A resolution. MurT has central and C-terminal domains similar to Mur ligases with a cysteine-rich insertion, which probably binds zinc, contributing to the interface with GatD. The mechanism of amidation by MurT is likely similar to the condensation catalyzed by Mur ligases. GatD is a glutaminase providing ammonia that is likely channeled to the MurT active site through a cavity network. The structure and assay presented here constitute a knowledge base for future drug development studies.

Structure of the essential peptidoglycan amidotransferase MurT/GatD complex from Streptococcus pneumoniae.,Morlot C, Straume D, Peters K, Hegnar OA, Simon N, Villard AM, Contreras-Martel C, Leisico F, Breukink E, Gravier-Pelletier C, Le Corre L, Vollmer W, Pietrancosta N, Havarstein LS, Zapun A Nat Commun. 2018 Aug 9;9(1):3180. doi: 10.1038/s41467-018-05602-w. PMID:30093673<ref>PMID:30093673</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6fqb" style="background-color:#fffaf0;"></div>

==See Also==
*[[Mur ligase|Mur ligase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus pneumoniae]]
[[Category: Breukink E]]
[[Category: Contreras-Martel C]]
[[Category: Gravier-Pelletier C]]
[[Category: Havarstein LS]]
[[Category: Hegnar OA]]
[[Category: Le Corre L]]
[[Category: Leisico F]]
[[Category: Morlot C]]
[[Category: Peters K]]
[[Category: Pietrancosta N]]
[[Category: Simon N]]
[[Category: Straume D]]
[[Category: Villard AM]]
[[Category: Vollmer W]]
[[Category: Zapun A]]