View source for 4ots


==Crystal Structure of isolated Operophtera brumata CPV18==
<StructureSection load='4ots' size='340' side='right'caption='[[4ots]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ots]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Operophtera_brumata_cypovirus_18 Operophtera brumata cypovirus 18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OTS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OTS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.704&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ots FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ots OCA], [https://pdbe.org/4ots PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ots RCSB], [https://www.ebi.ac.uk/pdbsum/4ots PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ots ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q30C70_9REOV Q30C70_9REOV] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
This work demonstrates that with the use of a microfocus synchrotron beam the structure of a novel viral polyhedrin could be successfully determined from microcrystals within cells, removing the preparatory step of sample isolation and maintaining a favourable biological environment. The data obtained are of high quality, comparable to that obtained from isolated crystals, and enabled a facile structure determination. A small but significant difference is observed between the unit-cell parameters and the mosaic spread of in cellulo and isolated crystals, suggesting that even these robust crystals are adversely affected by removal from the cell.

In cellulo structure determination of a novel cypovirus polyhedrin.,Axford D, Ji X, Stuart DI, Sutton G Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1435-41. doi:, 10.1107/S1399004714004714. Epub 2014 Apr 30. PMID:24816111<ref>PMID:24816111</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ots" style="background-color:#fffaf0;"></div>

==See Also==
*[[Polyhedrin|Polyhedrin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Operophtera brumata cypovirus 18]]
[[Category: Axford D]]
[[Category: Ji X]]
[[Category: Stuart DI]]
[[Category: Sutton GC]]