4ots

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Crystal Structure of isolated Operophtera brumata CPV18Crystal Structure of isolated Operophtera brumata CPV18

Structural highlights

4ots is a 1 chain structure with sequence from Operophtera brumata cypovirus 18. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.704Å
Ligands:, , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q30C70_9REOV

Publication Abstract from PubMed

This work demonstrates that with the use of a microfocus synchrotron beam the structure of a novel viral polyhedrin could be successfully determined from microcrystals within cells, removing the preparatory step of sample isolation and maintaining a favourable biological environment. The data obtained are of high quality, comparable to that obtained from isolated crystals, and enabled a facile structure determination. A small but significant difference is observed between the unit-cell parameters and the mosaic spread of in cellulo and isolated crystals, suggesting that even these robust crystals are adversely affected by removal from the cell.

In cellulo structure determination of a novel cypovirus polyhedrin.,Axford D, Ji X, Stuart DI, Sutton G Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1435-41. doi:, 10.1107/S1399004714004714. Epub 2014 Apr 30. PMID:24816111[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. Axford D, Ji X, Stuart DI, Sutton G. In cellulo structure determination of a novel cypovirus polyhedrin. Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1435-41. doi:, 10.1107/S1399004714004714. Epub 2014 Apr 30. PMID:24816111 doi:http://dx.doi.org/10.1107/S1399004714004714

4ots, resolution 1.70Å

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OCA