View source for 4l8q


==Crystal structure of Canavalia grandiflora seed lectin complexed with X-Man.==
<StructureSection load='4l8q' size='340' side='right'caption='[[4l8q]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4l8q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_grandiflora Canavalia grandiflora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L8Q FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l8q OCA], [https://pdbe.org/4l8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l8q RCSB], [https://www.ebi.ac.uk/pdbsum/4l8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l8q ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LECA_CANGR LECA_CANGR] Lectin (PubMed:24361256). Induces paw edema in mice (PubMed:22368061). Has a weak vasorelaxant effect on rat aorta (PubMed:24361256). Has anti-inflammatory and anti-nociceptive effects (PubMed:19153712).<ref>PMID:19153712</ref> <ref>PMID:22368061</ref> <ref>PMID:24361256</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lectins are comprised of a large family of proteins capable of the specific and reversible recognition of carbohydrates. Legume lectins, the most studied plant lectins, show high structural similarity, but with modifications that imply a variation in the intensity of some biological activities. In this work, the primary and tertiary structures of Canavalia grandiflora (ConGF) were determined. ConGF, a lectin isolated from C. grandiflora seeds, is able to induce relaxant activity in rat aortic rings. The complete sequence of ConGF comprises 237 amino acids. This particular protein has primary sequence variations commonly found in lectins from Dioclea and Canavalia genera. The protein structure was solved at 2.3 A resolution by X-ray crystallography. An X-Man molecule was modeled into the carbohydrate recognition domain. Still, ConGF (30 and 100 mug mL(-1)) elicited 25% of vasorelaxation (IC50=34.48 +/- 5.07 mug mL(-1)) in endothelialized aortic rings. A nonselective inhibitor of nitric oxide blocked ConGF relaxant effect, showing mediation by nitric oxide. Key distances between ConGF carbohydrate recognition domain residues were determined in order to explain this effect, in turn revealing some structural aspects that could differentiate lectins from the Canavalia genera with respect to different efficacy in vasorelaxant effect.

Vasorelaxant activity of Canavalia grandiflora seed lectin: A structural analysis.,Barroso-Neto IL, Simoes RC, Rocha BA, Bezerra MJ, Pereira-Junior FN, Silva Osterne VJ, Nascimento KS, Nagano CS, Delatorre P, Pereira MG, Freitas Pires A, Sampaio AH, Assreuy AM, Cavada BS Arch Biochem Biophys. 2014 Feb 1;543:31-9. doi: 10.1016/j.abb.2013.12.006. Epub, 2013 Dec 17. PMID:24361256<ref>PMID:24361256</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4l8q" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Canavalia grandiflora]]
[[Category: Large Structures]]
[[Category: Barroso-Neto IL]]
[[Category: Bezerra MJB]]
[[Category: Cavada BS]]
[[Category: Delatorre P]]
[[Category: Nagano CS]]
[[Category: Nascimento KS]]
[[Category: Osterne VJS]]
[[Category: Pereira-Junior FN]]
[[Category: Rocha BAM]]
[[Category: Sampaio AH]]
[[Category: Simoes RC]]