4l8q

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Crystal structure of Canavalia grandiflora seed lectin complexed with X-Man.Crystal structure of Canavalia grandiflora seed lectin complexed with X-Man.

Structural highlights

4l8q is a 1 chain structure with sequence from Canavalia grandiflora. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Ligands:, , , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LECA_CANGR Lectin (PubMed:24361256). Induces paw edema in mice (PubMed:22368061). Has a weak vasorelaxant effect on rat aorta (PubMed:24361256). Has anti-inflammatory and anti-nociceptive effects (PubMed:19153712).[1] [2] [3]

Publication Abstract from PubMed

Lectins are comprised of a large family of proteins capable of the specific and reversible recognition of carbohydrates. Legume lectins, the most studied plant lectins, show high structural similarity, but with modifications that imply a variation in the intensity of some biological activities. In this work, the primary and tertiary structures of Canavalia grandiflora (ConGF) were determined. ConGF, a lectin isolated from C. grandiflora seeds, is able to induce relaxant activity in rat aortic rings. The complete sequence of ConGF comprises 237 amino acids. This particular protein has primary sequence variations commonly found in lectins from Dioclea and Canavalia genera. The protein structure was solved at 2.3 A resolution by X-ray crystallography. An X-Man molecule was modeled into the carbohydrate recognition domain. Still, ConGF (30 and 100 mug mL(-1)) elicited 25% of vasorelaxation (IC50=34.48 +/- 5.07 mug mL(-1)) in endothelialized aortic rings. A nonselective inhibitor of nitric oxide blocked ConGF relaxant effect, showing mediation by nitric oxide. Key distances between ConGF carbohydrate recognition domain residues were determined in order to explain this effect, in turn revealing some structural aspects that could differentiate lectins from the Canavalia genera with respect to different efficacy in vasorelaxant effect.

Vasorelaxant activity of Canavalia grandiflora seed lectin: A structural analysis.,Barroso-Neto IL, Simoes RC, Rocha BA, Bezerra MJ, Pereira-Junior FN, Silva Osterne VJ, Nascimento KS, Nagano CS, Delatorre P, Pereira MG, Freitas Pires A, Sampaio AH, Assreuy AM, Cavada BS Arch Biochem Biophys. 2014 Feb 1;543:31-9. doi: 10.1016/j.abb.2013.12.006. Epub, 2013 Dec 17. PMID:24361256[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Nunes BS, Rensonnet NS, Dal-Secco D, Vieira SM, Cavada BS, Teixeira EH, Moura TR, Teixeira CS, Clemente-Napimoga JT, Cunha FQ, Napimoga MH. Lectin extracted from Canavalia grandiflora seeds presents potential anti-inflammatory and analgesic effects. Naunyn Schmiedebergs Arch Pharmacol. 2009 Jun;379(6):609-16. doi: , 10.1007/s00210-009-0397-9. Epub 2009 Jan 20. PMID:19153712 doi:http://dx.doi.org/10.1007/s00210-009-0397-9
  2. ↑ Simoes RC, Rocha BA, Bezerra MJ, Barroso-Neto IL, Pereira-Junior FN, da Mata Moura R, do Nascimento KS, Nagano CS, Delatorre P, de Freitas Pires A, Assreuy AM, Sampaio AH, Cavada BS. Protein crystal content analysis by mass spectrometry and preliminary X-ray diffraction of a lectin from Canavalia grandiflora seeds with modulatory role in inflammation. Rapid Commun Mass Spectrom. 2012 Apr 15;26(7):811-8. doi: 10.1002/rcm.6171. PMID:22368061 doi:http://dx.doi.org/10.1002/rcm.6171
  3. ↑ Barroso-Neto IL, Simoes RC, Rocha BA, Bezerra MJ, Pereira-Junior FN, Silva Osterne VJ, Nascimento KS, Nagano CS, Delatorre P, Pereira MG, Freitas Pires A, Sampaio AH, Assreuy AM, Cavada BS. Vasorelaxant activity of Canavalia grandiflora seed lectin: A structural analysis. Arch Biochem Biophys. 2014 Feb 1;543:31-9. doi: 10.1016/j.abb.2013.12.006. Epub, 2013 Dec 17. PMID:24361256 doi:http://dx.doi.org/10.1016/j.abb.2013.12.006
  4. ↑ Barroso-Neto IL, Simoes RC, Rocha BA, Bezerra MJ, Pereira-Junior FN, Silva Osterne VJ, Nascimento KS, Nagano CS, Delatorre P, Pereira MG, Freitas Pires A, Sampaio AH, Assreuy AM, Cavada BS. Vasorelaxant activity of Canavalia grandiflora seed lectin: A structural analysis. Arch Biochem Biophys. 2014 Feb 1;543:31-9. doi: 10.1016/j.abb.2013.12.006. Epub, 2013 Dec 17. PMID:24361256 doi:http://dx.doi.org/10.1016/j.abb.2013.12.006

4l8q, resolution 2.30Å

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