View source for 2of3


==TOG domain structure from C.elegans Zyg9==
<StructureSection load='2of3' size='340' side='right'caption='[[2of3]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2of3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OF3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2of3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2of3 OCA], [https://pdbe.org/2of3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2of3 RCSB], [https://www.ebi.ac.uk/pdbsum/2of3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2of3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ZYG9_CAEEL ZYG9_CAEEL] Plays a major role in organizing microtubules and spindle poles during mitosis and meiosis in one-cell stage embryos (PubMed:16971515). Required for default nucleus positioning in oocytes (PubMed:16971515).<ref>PMID:12956952</ref> <ref>PMID:15866166</ref> <ref>PMID:16054029</ref> <ref>PMID:16971515</ref> <ref>PMID:17666432</ref> <ref>PMID:2249759</ref> <ref>PMID:3949074</ref> <ref>PMID:6684994</ref> <ref>PMID:9606208</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/2of3_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2of3 ConSurf].
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== Publication Abstract from PubMed ==
Members of the XMAP215/Dis1 family of microtubule-associated proteins (MAPs) are essential for microtubule growth. MAPs in this family contain several 250 residue repeats, called TOG domains, which are thought to bind tubulin dimers and promote microtubule polymerization. We have determined the crystal structure of a single TOG domain from the Caenorhabditis elegans homolog, Zyg9, to 1.9 A resolution, and from it we describe a structural blueprint for TOG domains. These domains are flat, paddle-like structures, composed of six HEAT-repeat elements stacked side by side. The two wide faces of the paddle contain the HEAT-repeat helices, and the two narrow faces, the intra- and inter-HEAT repeat turns. Solvent-exposed residues in the intrarepeat turns are conserved, both within a particular protein and across the XMAP215/Dis1 family. Mutation of some of these residues in the TOG1 domain from the budding yeast homolog, Stu2p, shows that this face indeed participates in the tubulin contact.

Crystal structure of a TOG domain: conserved features of XMAP215/Dis1-family TOG domains and implications for tubulin binding.,Al-Bassam J, Larsen NA, Hyman AA, Harrison SC Structure. 2007 Mar;15(3):355-62. PMID:17355870<ref>PMID:17355870</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Caenorhabditis elegans]]
[[Category: Large Structures]]
[[Category: Al-Bassam J]]
[[Category: Harrison SC]]
[[Category: Hyman AA]]
[[Category: Larsen NA]]