TOG domain structure from C.elegans Zyg9TOG domain structure from C.elegans Zyg9
Structural highlights
2of3 is a 1 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
ZYG9_CAEEL Plays a major role in organizing microtubules and spindle poles during mitosis and meiosis in one-cell stage embryos (PubMed:16971515). Required for default nucleus positioning in oocytes (PubMed:16971515).[1][2][3][4][5][6][7][8][9]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Members of the XMAP215/Dis1 family of microtubule-associated proteins (MAPs) are essential for microtubule growth. MAPs in this family contain several 250 residue repeats, called TOG domains, which are thought to bind tubulin dimers and promote microtubule polymerization. We have determined the crystal structure of a single TOG domain from the Caenorhabditis elegans homolog, Zyg9, to 1.9 A resolution, and from it we describe a structural blueprint for TOG domains. These domains are flat, paddle-like structures, composed of six HEAT-repeat elements stacked side by side. The two wide faces of the paddle contain the HEAT-repeat helices, and the two narrow faces, the intra- and inter-HEAT repeat turns. Solvent-exposed residues in the intrarepeat turns are conserved, both within a particular protein and across the XMAP215/Dis1 family. Mutation of some of these residues in the TOG1 domain from the budding yeast homolog, Stu2p, shows that this face indeed participates in the tubulin contact.
Crystal structure of a TOG domain: conserved features of XMAP215/Dis1-family TOG domains and implications for tubulin binding.,Al-Bassam J, Larsen NA, Hyman AA, Harrison SC Structure. 2007 Mar;15(3):355-62. PMID:17355870[10]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑Srayko M, Quintin S, Schwager A, Hyman AA. Caenorhabditis elegans TAC-1 and ZYG-9 form a complex that is essential for long astral and spindle microtubules. Curr Biol. 2003 Sep 2;13(17):1506-11. PMID:12956952 doi:10.1016/s0960-9822(03)00597-9
↑Kimura A, Onami S. Computer simulations and image processing reveal length-dependent pulling force as the primary mechanism for C. elegans male pronuclear migration. Dev Cell. 2005 May;8(5):765-75. PMID:15866166 doi:10.1016/j.devcel.2005.03.007
↑Srayko M, Kaya A, Stamford J, Hyman AA. Identification and characterization of factors required for microtubule growth and nucleation in the early C. elegans embryo. Dev Cell. 2005 Aug;9(2):223-36. PMID:16054029 doi:10.1016/j.devcel.2005.07.003
↑DeBella LR, Hayashi A, Rose LS. LET-711, the Caenorhabditis elegans NOT1 ortholog, is required for spindle positioning and regulation of microtubule length in embryos. Mol Biol Cell. 2006 Nov;17(11):4911-24. PMID:16971515 doi:10.1091/mbc.e06-02-0107
↑Bellanger JM, Carter JC, Phillips JB, Canard C, Bowerman B, Gönczy P. ZYG-9, TAC-1 and ZYG-8 together ensure correct microtubule function throughout the cell cycle of C. elegans embryos. J Cell Sci. 2007 Aug 15;120(Pt 16):2963-73. PMID:17666432 doi:10.1242/jcs.004812
↑Mains PE, Kemphues KJ, Sprunger SA, Sulston IA, Wood WB. Mutations affecting the meiotic and mitotic divisions of the early Caenorhabditis elegans embryo. Genetics. 1990 Nov;126(3):593-605. PMID:2249759 doi:10.1093/genetics/126.3.593
↑Kemphues KJ, Wolf N, Wood WB, Hirsh D. Two loci required for cytoplasmic organization in early embryos of Caenorhabditis elegans. Dev Biol. 1986 Feb;113(2):449-60. PMID:3949074 doi:10.1016/0012-1606(86)90180-6
↑Strome S, Wood WB. Generation of asymmetry and segregation of germ-line granules in early C. elegans embryos. Cell. 1983 Nov;35(1):15-25. PMID:6684994 doi:10.1016/0092-8674(83)90203-9
↑Matthews LR, Carter P, Thierry-Mieg D, Kemphues K. ZYG-9, a Caenorhabditis elegans protein required for microtubule organization and function, is a component of meiotic and mitotic spindle poles. J Cell Biol. 1998 Jun 1;141(5):1159-68. PMID:9606208 doi:10.1083/jcb.141.5.1159
↑Al-Bassam J, Larsen NA, Hyman AA, Harrison SC. Crystal structure of a TOG domain: conserved features of XMAP215/Dis1-family TOG domains and implications for tubulin binding. Structure. 2007 Mar;15(3):355-62. PMID:17355870 doi:10.1016/j.str.2007.01.012
