View source for 1jpl

[[Image:1jpl.gif|left|200px]]<br />
<applet load="1jpl" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1jpl, resolution 2.40&Aring;" />
'''GGA3 VHS domain complexed with C-terminal peptide from cation-independent mannose 6-phosphate receptor'''<br />

==Overview==
Specific sorting signals direct transmembrane proteins to the compartments, of the endosomal-lysosomal system. Acidic-cluster-dileucine signals, present within the cytoplasmic tails of sorting receptors, such as the, cation-independent and cation-dependent mannose-6-phosphate receptors, are, recognized by the GGA (Golgi-localized, gamma-ear-containing, ADP-ribosylation-factor-binding) proteins. The VHS (Vps27p, Hrs and STAM), domains of the GGA proteins are responsible for the highly specific, recognition of these acidic-cluster-dileucine signals. Here we report the, structures of the VHS domain of human GGA3 complexed with signals from, both mannose-6-phosphate receptors. The signals bind in an extended, conformation to helices 6 and 8 of the VHS domain. The structures, highlight an Asp residue separated by two residues from a dileucine, sequence as critical recognition elements. The side chains of the, Asp-X-X-Leu-Leu sequence interact with subsites consisting of one, electropositive and two shallow hydrophobic pockets, respectively. The, rigid spatial alignment of the three binding subsites leads to high, specificity.

==Disease==
Known diseases associated with this structure: Hepatocellular carcinoma OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147280 147280]]

==About this Structure==
1JPL is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JPL OCA]. 

==Reference==
Structural basis for acidic-cluster-dileucine sorting-signal recognition by VHS domains., Misra S, Puertollano R, Kato Y, Bonifacino JS, Hurley JH, Nature. 2002 Feb 21;415(6874):933-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11859375 11859375]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Bonifacino, J.S.]]
[[Category: Hurley, J.H.]]
[[Category: Misra, S.]]
[[Category: Puertollano, R.]]
[[Category: protein-peptide complex; vhs domain; dxxll sorting signal]]

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