View source for 4wsb


==Bat Influenza A polymerase with bound vRNA promoter==
<StructureSection load='4wsb' size='340' side='right'caption='[[4wsb]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4wsb]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/little_yellow-shouldered_bat/Guatemala/060/2010(H17N10)) Influenza A virus (A/little yellow-shouldered bat/Guatemala/060/2010(H17N10))] and [https://en.wikipedia.org/wiki/Unidentified_influenza_virus Unidentified influenza virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WSB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WSB FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wsb OCA], [https://pdbe.org/4wsb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wsb RCSB], [https://www.ebi.ac.uk/pdbsum/4wsb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wsb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/H6QM90_9INFA H6QM90_9INFA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Influenza virus polymerase uses a capped primer, derived by 'cap-snatching' from host pre-messenger RNA, to transcribe its RNA genome into mRNA and a stuttering mechanism to generate the poly(A) tail. By contrast, genome replication is unprimed and generates exact full-length copies of the template. Here we use crystal structures of bat influenza A and human influenza B polymerases (FluA and FluB), bound to the viral RNA promoter, to give mechanistic insight into these distinct processes. In the FluA structure, a loop analogous to the priming loop of flavivirus polymerases suggests that influenza could initiate unprimed template replication by a similar mechanism. Comparing the FluA and FluB structures suggests that cap-snatching involves in situ rotation of the PB2 cap-binding domain to direct the capped primer first towards the endonuclease and then into the polymerase active site. The polymerase probably undergoes considerable conformational changes to convert the observed pre-initiation state into the active initiation and elongation states.

Structural insight into cap-snatching and RNA synthesis by influenza polymerase.,Reich S, Guilligay D, Pflug A, Malet H, Berger I, Crepin T, Hart D, Lunardi T, Nanao M, Ruigrok RW, Cusack S Nature. 2014 Nov 19. doi: 10.1038/nature14009. PMID:25409151<ref>PMID:25409151</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4wsb" style="background-color:#fffaf0;"></div>

==See Also==
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Unidentified influenza virus]]
[[Category: Cusack S]]
[[Category: Guilligay D]]
[[Category: Pflug A]]
[[Category: Reich S]]