View source for 3gkl


==Following evolutionary paths to high affinity and selectivity protein-protein interactions using Colicin7 and Immunity proteins==
<StructureSection load='3gkl' size='340' side='right'caption='[[3gkl]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3gkl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GKL FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3gjn|3gjn]]</div></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">colE7, cea ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), imm, ceiE9 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gkl OCA], [https://pdbe.org/3gkl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gkl RCSB], [https://www.ebi.ac.uk/pdbsum/3gkl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gkl ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/CEA7_ECOLX CEA7_ECOLX]] This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity.  Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria. [[https://www.uniprot.org/uniprot/IMM9_ECOLX IMM9_ECOLX]] This protein is able to protect a cell, which harbors the plasmid ColE9 encoding colicin E9, against colicin E9, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gk/3gkl_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gkl ConSurf].
<div style="clear:both"></div>

==See Also==
*[[Colicin 3D structures|Colicin 3D structures]]
*[[Colicin Immunity Protein|Colicin Immunity Protein]]
*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
__TOC__
</StructureSection>
[[Category: Bacillus coli migula 1895]]
[[Category: Large Structures]]
[[Category: Dym, O]]
[[Category: ISPC, Israel Structural Proteomics Center]]
[[Category: Tawfik, D S]]
[[Category: Antibiotic]]
[[Category: Antimicrobial]]
[[Category: Bacteriocin]]
[[Category: Bacteriocin immunity]]
[[Category: Endonuclease]]
[[Category: Hydrolase]]
[[Category: Metal-binding]]
[[Category: Nuclease]]
[[Category: Plasmid]]
[[Category: Protein-protein complex]]
[[Category: Structural genomic]]
[[Category: Zinc]]