View source for 5elb


==Cholera toxin classical B-pentamer in complex with Lewis-y==
<StructureSection load='5elb' size='340' side='right' caption='[[5elb]], [[Resolution|resolution]] 1.08&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5elb]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ELB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ELB FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCN:BICINE'>BCN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5elb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5elb OCA], [http://pdbe.org/5elb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5elb RCSB], [http://www.ebi.ac.uk/pdbsum/5elb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5elb ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cholera is the prime example of blood-group-dependent diseases, with individuals of blood group O experiencing the most severe symptoms. The cholera toxin is the main suspect to cause this relationship. We report the high-resolution crystal structures (1.1-1.6 A) of the native cholera toxin B-pentamer for both classical and El Tor biotypes, in complexes with relevant blood group determinants and a fragment of its primary receptor, the GM1 ganglioside. The blood group A determinant binds in the opposite orientation compared to previously published structures of the cholera toxin, whereas the blood group H determinant, characteristic of blood group O, binds in both orientations. H-determinants bind with higher affinity than A-determinants, as shown by surface plasmon resonance. Together, these findings suggest why blood group O is a risk factor for severe cholera.

High-Resolution Crystal Structures Elucidate the Molecular Basis of Cholera Blood Group Dependence.,Heggelund JE, Burschowsky D, Bjornestad VA, Hodnik V, Anderluh G, Krengel U PLoS Pathog. 2016 Apr 15;12(4):e1005567. doi: 10.1371/journal.ppat.1005567., eCollection 2016 Apr. PMID:27082955<ref>PMID:27082955</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5elb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Burschowsky, D]]
[[Category: Heggelund, J E]]
[[Category: Krengel, U]]
[[Category: Blood group oligosaccharide/antigen]]
[[Category: Cholera toxin b-pentamer]]
[[Category: Complex]]
[[Category: Lewis-y]]
[[Category: Toxin]]