View source for 2nsf

[[Image:2nsf.gif|left|200px]]<br /><applet load="2nsf" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2nsf, resolution 1.750&Aring;" />
'''Crystal structure of the mycothiol-dependent maleylpyruvate isomerase'''<br />

==Overview==
Mycothiol (MSH) is the major low molecular mass thiols in many Gram-positive bacteria such as Mycobacterium tuberculosis and Corynebacterium glutamicum. The physiological roles of MSH are believed to be equivalent to those of GSH in Gram-negative bacteria, but current knowledge of MSH is limited to detoxification of alkalating chemicals and protection from host cell defense/killing systems. Recently, an MSH-dependent maleylpyruvate isomerase (MDMPI) was discovered from C. glutamicum, and this isomerase represents one example of many putative MSH-dependent enzymes that take MSH as cofactor. In this report, fourteen mutants of MDMPI were generated. The wild type and mutant (H52A) MDMPIs were crystallized and their structures were solved at 1.75 and 2.05 A resolution, respectively. The crystal structures reveal that this enzyme contains a divalent metal-binding domain and a C-terminal domain possessing a novel folding pattern (alphabetaalphabetabetaalpha fold). The divalent metal-binding site is composed of residues His52, Glu144, and His148 and is located at the bottom of a surface pocket. Combining the structural and site-directed mutagenesis studies, it is proposed that this surface pocket including the metal ion and MSH moiety formed the putative catalytic center.

==About this Structure==
2NSF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Maleylpyruvate_isomerase Maleylpyruvate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.4 5.2.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NSF OCA]. 

==Reference==
Crystal structures and site-directed mutagenesis of a mycothiol-dependent enzyme reveal a novel folding and molecular basis for mycothiol-mediated maleylpyruvate isomerization., Wang R, Yin YJ, Wang F, Li M, Feng J, Zhang HM, Zhang JP, Liu SJ, Chang WR, J Biol Chem. 2007 Jun 1;282(22):16288-294. Epub 2007 Apr 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17428791 17428791]
[[Category: Corynebacterium glutamicum]]
[[Category: Maleylpyruvate isomerase]]
[[Category: Single protein]]
[[Category: Chang, W R.]]
[[Category: Wang, R.]]
[[Category: GOL]]
[[Category: SO4]]
[[Category: ZN]]
[[Category: metal binding]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:10:24 2008''