View source for 1s60

[[Image:1s60.jpg|left|200px]]<br /><applet load="1s60" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="1s60, resolution 3.0&Aring;" />
'''Aminoglycoside N-Acetyltransferase AAC(6')-Iy in Complex with CoA and N-terminal His(6)-tag (crystal form 2)'''<br />

==Overview==
The Salmonella enterica chromosomally encoded AAC(6')-Iy has been shown to confer broad aminoglycoside resistance in strains in which the structural gene is expressed. The three-dimensional structures reported place the enzyme in the large Gcn5-related N-acetyltransferase (GNAT) superfamily. The structure of the CoA-ribostamycin ternary complex allows us to propose a chemical mechanism for the reaction, and comparison with the Mycobacterium tuberculosis AAC(2')-CoA-ribostamycin complex allows us to define how regioselectivity of acetylation is achieved. The AAC(6')-Iy dimer is most structurally similar to the Saccharomyces cerevisiae Hpa2-encoded histone acetyltransferase. We demonstrate that AAC(6')-Iy catalyzes both acetyl-CoA-dependent self-alpha-N-acetylation and acetylation of eukaryotic histone proteins and the human histone H3 N-terminal peptide. These structural and catalytic similarities lead us to propose that chromosomally encoded bacterial acetyltransferases, including those functionally identified as aminoglycoside acetyltransferases, are the evolutionary progenitors of the eukaryotic histone acetyltransferases.

==About this Structure==
1S60 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_enteritidis Salmonella enteritidis] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=COA:'>COA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aminoglycoside_N(6')-acetyltransferase Aminoglycoside N(6')-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.82 2.3.1.82] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S60 OCA]. 

==Reference==
A bacterial acetyltransferase capable of regioselective N-acetylation of antibiotics and histones., Vetting MW, Magnet S, Nieves E, Roderick SL, Blanchard JS, Chem Biol. 2004 Apr;11(4):565-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15123251 15123251]
[[Category: Aminoglycoside N(6')-acetyltransferase]]
[[Category: Salmonella enteritidis]]
[[Category: Single protein]]
[[Category: Blanchard, J S.]]
[[Category: Magnet, S.]]
[[Category: Nieves, E.]]
[[Category: Roderick, S L.]]
[[Category: Vetting, M W.]]
[[Category: COA]]
[[Category: SO4]]
[[Category: acetyltransferase]]
[[Category: aminoglycoside]]
[[Category: coa]]
[[Category: gnat]]
[[Category: n-acetyltransferase]]

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