View source for 8qzu


==XhpG hydrolase mutant S98A of Xenorhabdus hominickii==
<StructureSection load='8qzu' size='340' side='right'caption='[[8qzu]], [[Resolution|resolution]] 1.18&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8qzu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenorhabdus_hominickii Xenorhabdus hominickii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QZU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.18&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qzu OCA], [https://pdbe.org/8qzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qzu RCSB], [https://www.ebi.ac.uk/pdbsum/8qzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qzu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2G0QDP0_9GAMM A0A2G0QDP0_9GAMM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pyrrolizidine alkaloids (PAs) are a structurally diverse group of heterocyclic specialized metabolites characterized by a core structure comprising a hexahydro-1H-pyrrolizine. PAs are synthesized through two main pathways. In plants, assembly occurs via a homospermidine synthase, and in bacteria, through combined action of a nonribosomal peptide synthetase and a Baeyer-Villiger monooxygenase. While the toxic properties of plant-derived PAs and their prevalence in animal and human foods have been extensively studied, the biological roles and biosynthesis of more complex bacterial PAs are not well understood. Here, we report the identification and characterization of a bacterial biosynthetic gene cluster from Xenorhabdus hominickii, xhpA-G, which is responsible for producing the PA pseudo-dimer pyrrolizwilline. Analysis of X. hominickii promoter exchange mutants together with heterologous expression of xhpA-G in E. coli, revealed a set of pathway intermediates, two of which were chemically synthesized, as well as multiple derivatives. This information was leveraged to propose a detailed biosynthetic pathway to pyrrolizwilline. Furthermore, we have characterized the hydrolase XhpG, the key enzyme in the conversion of the pathway intermediate pyrrolizixenamide to pyrrolizwilline, using X-ray crystallography and small-angle X-ray scattering (SAXS).

Pyrrolizwilline, a unique bacterial alkaloid assembled by a nonribosomal peptide synthetase and non-enzymatic dimerization.,Effert J, Westphalen M, Calderari A, Shi YM, Elamri I, Najah S, Grun P, Li Y, Gruez A, Weissman KJ, Bode HB Angew Chem Int Ed Engl. 2024 Oct 20:e202411258. doi: 10.1002/anie.202411258. PMID:39428351<ref>PMID:39428351</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8qzu" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Xenorhabdus hominickii]]
[[Category: Calderari A]]
[[Category: Gruez A]]
[[Category: Weissman KJ]]