View source for 3vvt


==Crystal structure of reconstructed archaeal ancestral NDK, Arc1==
<StructureSection load='3vvt' size='340' side='right'caption='[[3vvt]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3vvt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VVT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VVT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vvt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vvt OCA], [https://pdbe.org/3vvt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vvt RCSB], [https://www.ebi.ac.uk/pdbsum/3vvt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vvt ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Theoretical studies have focused on the environmental temperature of the universal common ancestor of life with conflicting conclusions. Here we provide experimental support for the existence of a thermophilic universal common ancestor. We present the thermal stabilities and catalytic efficiencies of nucleoside diphosphate kinases (NDK), designed using the information contained in predictive phylogenetic trees, that seem to represent the last common ancestors of Archaea and of Bacteria. These enzymes display extreme thermal stabilities, suggesting thermophilic ancestries for Archaea and Bacteria. The results are robust to the uncertainties associated with the sequence predictions and to the tree topologies used to infer the ancestral sequences. Moreover, mutagenesis experiments suggest that the universal ancestor also possessed a very thermostable NDK. Because, as we show, the stability of an NDK is directly related to the environmental temperature of its host organism, our results indicate that the last common ancestor of extant life was a thermophile that flourished at a very high temperature.

Experimental evidence for the thermophilicity of ancestral life.,Akanuma S, Nakajima Y, Yokobori S, Kimura M, Nemoto N, Mase T, Miyazono K, Tanokura M, Yamagishi A Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):11067-72. doi:, 10.1073/pnas.1308215110. Epub 2013 Jun 17. PMID:23776221<ref>PMID:23776221</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3vvt" style="background-color:#fffaf0;"></div>

==See Also==
*[[Nucleoside diphosphate kinase 3D structures|Nucleoside diphosphate kinase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Akanuma S]]
[[Category: Kimura M]]
[[Category: Miyazono K]]
[[Category: Nemoto N]]
[[Category: Tanokura M]]
[[Category: Yamagishi A]]
[[Category: Yokobori S]]