View source for 3ign


==Crystal Structure of the GGDEF domain from Marinobacter aquaeolei diguanylate cyclase complexed with c-di-GMP - Northeast Structural Genomics Consortium Target MqR89a==
<StructureSection load='3ign' size='340' side='right'caption='[[3ign]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ign]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Marinobacter_nauticus_VT8 Marinobacter nauticus VT8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IGN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.83&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C2E:9,9-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d 3,2-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)'>C2E</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ign FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ign OCA], [https://pdbe.org/3ign PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ign RCSB], [https://www.ebi.ac.uk/pdbsum/3ign PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ign ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A1U3W3_MARN8 A1U3W3_MARN8] 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ig/3ign_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ign ConSurf].
<div style="clear:both"></div>
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== Publication Abstract from PubMed ==
Recent studies of signal transduction in bacteria have revealed a unique second messenger, bis-(3'-5')-cyclic dimeric GMP (c-di-GMP), which regulates transitions between motile states and sessile states, such as biofilms. C-di-GMP is synthesized from two GTP molecules by diguanylate cyclases (DGC). The catalytic activity of DGCs depends on a conserved GG(D/E)EF domain, usually part of a larger multi-domain protein organization. The domains other than the GG(D/E)EF domain often control DGC activation. This paper presents the 1.83 A crystal structure of an isolated catalytically competent GG(D/E)EF domain from the A1U3W3_MARAV protein from Marinobacter aquaeolei. Co-crystallization with GTP resulted in enzymatic synthesis of c-di-GMP. Comparison with previously solved DGC structures shows a similar orientation of c-di-GMP bound to an allosteric regulatory site mediating feedback inhibition of the enzyme. Biosynthesis of c-di-GMP in the crystallization reaction establishes that the enzymatic activity of this DGC domain does not require interaction with regulatory domains.

Crystal structure of a catalytically active GG(D/E)EF diguanylate cyclase domain from Marinobacter aquaeolei with bound c-di-GMP product.,Vorobiev SM, Neely H, Yu B, Seetharaman J, Xiao R, Acton TB, Montelione GT, Hunt JF J Struct Funct Genomics. 2012 Sep;13(3):177-83. doi: 10.1007/s10969-012-9136-4., Epub 2012 Jul 29. PMID:22843345<ref>PMID:22843345</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3ign" style="background-color:#fffaf0;"></div>

==See Also==
*[[Diguanylate cyclase|Diguanylate cyclase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Marinobacter nauticus VT8]]
[[Category: Acton TB]]
[[Category: Ciccosanti C]]
[[Category: Foote EL]]
[[Category: Hunt JF]]
[[Category: Montelione GT]]
[[Category: Neely H]]
[[Category: Sahdev S]]
[[Category: Seetharaman J]]
[[Category: Tong L]]
[[Category: Vorobiev S]]
[[Category: Wang H]]
[[Category: Xiao R]]