User:Jaime Prilusky/Guide/Structure Function Med
Guide for students:
To perform the proposed activity students enter the following Web sites and answer the following questions related to proteins.
Hemoglobin and Myoglobin: http://Proteopedia.org/w/Hemoglobin
a) Identify the chains that form hemoglobin and myoglobin, what kind of secondary, tertiary, and quaternary structure can you recognize? . Mention the differences between these two proteins b) Identify the prosthetic group, iron (Fe) and oxygen binding site in both proteins. c) Return to hemoglobin structure and observe the location of the histidine f9 d) Superimposing the cursor on the structure permits different amino acids to be labeled. Analyze the type of amino acids that you find within the structure and justify their locations. e) Using the tool “evolutionary conservation” you can observe which amino acids have been conserved or which ones have varied. In what area do you find the most conserved amino acids in hemoglobin structure? Elaborate on this conclusion. f) Identify an amino acid change which leads to anomalous hemoglobin
Iron Metabolism Protein: http://proteopedia.org/w/Sandbox_116_-_Human_Hemochromatosis_Protein_(1a6z)
a) Login to the site of HFE (Human Hemochromatosis Protein) Sandbox 106 . Describe its role and structure b) HFE has a high homology with other protein, which protein? In what they differ? c) HFE can bind β2-microglobulin, find this interaction in the structure and mention the diverse type of secondary structure you can differentiate. d) Identify within the structure the Transferrin Receptor (RTf) and the aminoacids that interact with HFE e) What kind of disease is Hemochromatosis?. Identify different mutations leading to this pathology
Immune System Protein: http://Proteopedia.org/w/Immunoglobulin
a) Login to the site of immunoglobulins (Ig). Identify: Chains, secondary, tertiary and quaternary structure, carbohydrate region, hinge region, heavy chain domain, variable and hypervariable region, Fab region, Fc, Fab-epitope formation of an antigen (Ag) complex. b) Scroll down to the section on secretory IgAs. Identify all possible structures of this Ig.
Fibrous Proteins: Collagen http://www.proteopedia.org/w/Collagen Login to the site of collagen a) What is the function of this protein? b) Which amino acids predominate in the structure? Is there little or great variability of amino acids? c) List the main characteristics of the secondary structure of this protein d) What would happen if a mutation occurs in the polypeptide chain that changes a glycine to an alanine?