Topoisomerase binding protein
<StructureSection load= size='350' side='right' caption='Structure of human topoisomerase binding protein 1 BRCT 4-5 domain complex with phospho-peptide (magenta) (PDB entry 3ueo)' scene='56/564070/Cv/4'>
FunctionFunction
Topoisomerase binding protein or DNA topoisomerase 2-binding protein 1 (TopBP1) interacts with the C-terminal of topoisomerase II beta. TopBP1 is required for DNA replication. It binds double-stranded DNA breaks and nicks and plays a role in rescuing stalled DNA replication forks[1].
Structural highlightsStructural highlights
TopBP1 contains BRCT domains which are ca. 100 amino acid long tandem repeat which bind phosphor-peptides. The [2].
3D structures of topoisomerase binding protein3D structures of topoisomerase binding protein
Updated on 27-April-2022
Domains - BRCT 0-2 1-287; BRCT 4-5 549-746; BRCT 6 893-996; BRCT 7-8 1264-1493
2xnh, 2xnk – hTopBP1 BRCT 0-2 domains - human
6hm5 – hTopBP1 BRCT 0-2 domains + Rad9
6rml – hTopBP1 BRCT 0-2 domains + 53bp1
3olc – hTopBP1 N terminal
3uen – hTopBP1 BRCT 4-5 domains
3ueo, 5u6k, 6rmm – hTopBP1 BRCT 4-5 domains + phosphor-peptide
5u6k – hTopBP1 BRCT 4-5 domains + BLM
3jve, 3pd7 – hTopBP1 BRCT 6 domain
3al2 – hTopBP1 BRCT 7-8 domains
3al3 – hTopBP1 BRCT 7-8 domains + Fanconi anemia group J protein peptide
7cmz – hTopBP1 BRCT 7-8 domains + PHF8 peptide
ReferencesReferences
- ↑ Yamane K, Kawabata M, Tsuruo T. A DNA-topoisomerase-II-binding protein with eight repeating regions similar to DNA-repair enzymes and to a cell-cycle regulator. Eur J Biochem. 1997 Dec 15;250(3):794-9. PMID:9461304
- ↑ Leung CC, Sun L, Gong Z, Burkat M, Edwards R, Assmus M, Chen J, Glover JN. Structural insights into recognition of MDC1 by TopBP1 in DNA replication checkpoint control. Structure. 2013 Aug 6;21(8):1450-9. doi: 10.1016/j.str.2013.06.015. Epub 2013 Jul, 25. PMID:23891287 doi:http://dx.doi.org/10.1016/j.str.2013.06.015