Sandbox 1mel
Dromedary NanobodyDromedary Nanobody
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| 1mel, resolution 2.50Å () | |||||||||
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| Gene: | ANTIBODY VH FRAGMENT CAB-LYS3 (Camelus dromedarius), ANTIBODY VH FRAGMENT CAB-LYS3 (Gallus gallus) | ||||||||
| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
CRYSTAL STRUCTURE OF A CAMEL SINGLE-DOMAIN VH ANTIBODY FRAGMENT IN COMPLEX WITH LYSOZYMECRYSTAL STRUCTURE OF A CAMEL SINGLE-DOMAIN VH ANTIBODY FRAGMENT IN COMPLEX WITH LYSOZYME
The Camelidae is the only taxonomic family known to possess functional heavy-chain antibodies, lacking light chains. We report here the 2.5 A resolution crystal structure of a camel VH in complex with its antigen, lysozyme. Compared to human and mouse VH domains, there are no major backbone rearrangements in the VH framework. However, the architecture of the region of VH that interacts with a VL in a conventional FV is different from any previously seen. Moreover, the CDR1 region, although in sequence homologous to human CDR1, deviates fundamentally from the canonical structure. Additionally, one half of the CDR3 contacts the VH region which in conventional immunoglobulins interacts with a VL whereas the other half protrudes from the antigen binding site and penetrates deeply into the active site of lysozyme.
Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme., Desmyter A, Transue TR, Ghahroudi MA, Thi MH, Poortmans F, Hamers R, Muyldermans S, Wyns L, Nat Struct Biol. 1996 Sep;3(9):803-11. PMID:8784355
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this StructureAbout this Structure
1mel is a 4 chain structure with sequence from Camelus dromedarius and Gallus gallus. The April 2011 RCSB PDB Molecule of the Month feature on Nanobodies by David Goodsell is 10.2210/rcsb_pdb/mom_2011_4. Full crystallographic information is available from OCA.