Peptide N-glycanase

Function Peptide N-glycanase (PNG) cleaves oligosaccharides from misfolded proteins and prepares them for degradation. PNG cleaves at β-aspartyl glucosamine bond to remove a glycan moiety from glycoprotein substrate. PNG is present in prokaryotes and eukaryotes. PNG N terminal PUB domain interacts with the AAA ATPase protein p97 which is a central component in the ubiquitin-proteasome system. Peptide N-glycanase F cleaves Asn-GlcNac bond in N-linked glycoproteins and glycopeptides. Disease Mutations in human PNG genes were found in patients showing developmental delay, multifocal epilepsy and other multiple symptoms. Relevance PNG F is used in studying of glycoproteins. Structural highlights . .

spinqualitylabelsall models Yeast PNGase catalytic domain (sky blue) complex with Rad23 (green), Zn+2 (grey) and Cl- (green) ions (PDB code 2f4m). Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D Structures of PNGase3D Structures of PNGase

Updated on 22-August-2023

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• PNGase
  • 2ccq – hPNG PUB domain – human
    
  • 2cm0 – hPNG PUB domain (mutant)
    
  • 2g9f, 2g9g – mPNG C terminal – mouse
    
  • 2hpj – mPNG PUB domain
    
  • 2d5u – mPNG PUB domain - NMR
    
  • 2hpl – mPNG PUB domain + peptide
    
  • 2i74 – mPNG C terminal + mannose
    
  • 1x3w – yPNG + Rad23 - yeast
    
  • 3esw – yPNG + Rad23 + GlcNac
    
  • 1x3z – yPNG + Rad23 + peptide
    
  • 2f4m – yPNG catalytic domain + Rad23
    
  • 2f4o – mPNG catalytic domain+ HR23 + peptide
    
• PNGase F
  • 1pgs, 1png, 3pms – EmPNG – Elizabethkingia meningoseptica
    
  • 4r4x, 4r4z – EmPNG-II
    
  • 1pnf – EmPNG + acetylchitobiose