Mur ligase

Function Mur ligase are a set of four Mur ubiquitin ligase enzymes: MurC, MurD, MurE, MurF which catalyze the addition of a short polypeptide to UDP-D-acetylmuramic acid in the process of bacterial cell wall buildup from peptidoglycans. All four enzymes are topologically similar. MurC ligase or UDP-N-acetylmuramoyl-L-alanine ligase adds L-alanine to peptidoglycans. MurD ligase or UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase catalyzes the conversion of UDP-N-acetylmuramoyl-L-alanine (UMA), D-glutamate and ATP to UDP-N-acetylmuramoyl-L-alanine-D-glutamate and ADP. I.e. adds D-glutamate to peptidoglycans. MurE ligase or UDP-N-acetylmuramoyl-L-alaninyl-D-glutamate-2,6-diaminopimelate ligase adds meso-diaminpimelate to peptidoglycans. MurF ligase or UDP-N-acetylmuramoyl-L-alanine-D-glutamyl-lysine-D-alanyl-D-alanine ligase adds D-alanyl-D-alanine to peptidoglycans. Relevance Mur ligases are antibacterial drug targets. Structural highlights . . . C-terminal domain binds the incorporated amino acid. Water molecules are shown as red spheres. ,

spinqualitylabelsall models Structure of MurD ligase complex with UMA, ADP and Mn+2 ion (purple) (PDB entry 3uag) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D Structures of Mur ligase3D Structures of Mur ligase

Updated on 10-November-2021

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• MurD or UDP-N-acetylmuramic acid L-alanyl-D-glutmate ligase
  • 1e0d, 5a5e – EcMurD – Escherichia coli
    
  • 3lk7 – MurD – Streptococcus agalactiae
    
  • 4buc – TmMurD – Thermotoga maritima
    
• MurD complex with ligand
  • 1uag, 1eeh – EcMurD + UMA
    
  • 2jfg – EcMurD + UMA + ADP
    
  • 2uag – EcMurD + UMA + ADP + Mg
    
  • 3uag – EcMurD + UMA + ADP + Mn
    
  • 5a5f – EcMurD + UMA + ADP + malonate
    
  • 4uag – EcMurD + UAG
    
  • 4bub – TmMurD + ADP
    
• MurD complex with inhibitor
  • 2jff, 2jfh, 2uuo, 2uup, 2vtd, 2vte, 2xpc – EcMurD + sulfonamide inhibitor
    
  • 2wjp – EcMurD + rhodanine inhibitor
    
  • 2x5o, 2y68, 2y66, 2y67, 2y1o – EcMurD + thiazolidine inhibitor
    
• MurC or UDP-N-acetylmuramic acid L-alanine ligase
  • 1j6u – TmMurC
    
  • 7bva – MtMurC – Mycobacterium tuberculosis
    
  • 7bvb – MtMurC + UAG
    
  • 6cau – AbMurC + AMPPNP – Acinetobacter baumannii
    
  • 6x9f, 6x9n – MurC + inhibitor – Pseudomonas aeruginosa
    
  • 6vr7 – MfMurC – Methanothermus fervidus
    
• MurE or UDP-N-acetylmuramic acid L-alanyl-D-glutmate-2,6-diaminopimelate ligase
  • 2xja – MtMurE + dipeptide + ADP – Mycobacterium tuberculosis
    
  • 2wtz – MtMurE + UAG
    
  • 7b53 – EcMurE
    
  • 7b6o – EcMurE (mutant)
    
  • 1e8c – EcMurE + UDP-tripeptide + UAG
    
  • 7b60, 7b61, 7b68, 7b6g, 7b6i, 7b6j, 7b6l, 7b6k, 7b6m, 7b6n, 7b6q, 7b9e, 7b9w – EcMurE + inhibitor
    
  • 7b6p – EcMurE (mutant) + inhibitor
    
  • 4c12 – SaMurE + UML + ADP – Staphylococcus aureus
    
  • 4c13 – SaMurE + UML
    
  • 7jt8 – MfMurE
    
  • 6vr8 – MfMurE + UDP
    
  • 7d27, 7sir – AbMurE
    
• MurF
  • 3ddt – hMurF-1 ZF-B box + Zn – human
    
  • 2d8u – hMurF-1 ZF-B box + Zn - NMR
    
  • 3q1d – hMurF-3
    
  • 4m3l – hMurF-1 coiled coil domain
    
  • 2am1, 2am2, 3zm5, 3zm6 – SpMurF + inhibitor – Streptococcus pneumoniae
    
  • 3zl8 – TmMurF + ADP
    
  • 1gg4 – EcMurF
    
  • 4qdi, 4qf5, 4ziy – MurF - Acinetobacter baumannii
    
  • 4cvl – PaMurF + AMPPNP - Pseudomonas aeruginosa
    
  • 4cvk – PaMurF + UDP-MurNac
    
  • 4cvm – PaMurF + UDP-MurNac + AMPPNP
    
• MurT
  • 6fqb – SpMurT + glutamine amidotransferase
    
  • 6gs2 – SaMurT + glutamine amidotransferase
    
  • 6h5e – SaMurT + glutamine amidotransferase + AMPPNP