Glucanase

Glucanases (Gase) are enzymes which break down glucans which are part of plant cell wall. Gase hydrolyzes the 1,4-β-glycosidic link in cellulose, lichenin and β-D-glucan.

Endo-Gase breaks internal cellulose bonds^[1].
Exo-Gase cleaves 2 to 4 units from the ends of exposed cellulose polysaccharide chains^[2]. *Xyloglucanase (XGase) breaks down the hemicellulose xyloglucan^[3].
alpha-1,3-glucanase hydrolyzes alpha-1,3-glucan ^[4].
beta-1,3-glucanase enhances fungal resistance in plants^[5].

Gase are classified according to polysaccharide bond which they break. For details see
*Molecular Playground/1,3-1,4-beta-D-glucanase
*Crystal Structure Determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A
*(1→3)-α-Glucanases: An Alternative to Control Dental Plaque and Improve Oral Health.

3D structures of glucanase

Glucanase 3D structures

Endo-1,3-β-glucanase complex with glycerol, phosphate and Ca+2 ion, 3atg

Drag the structure with the mouse to rotate

ReferencesReferences

↑ Barras DR, Stone BA. Beta-1,3-glucan hydrolases from Euglena gracilis. I. The nature of the hydrolases. Biochim Biophys Acta. 1969 Nov 4;191(2):329-41. PMID:5354264
↑ Tsai LC, Amiraslanov I, Chen HR, Chen YW, Lee HL, Liang PH, Liaw YC. Structures of exoglucanase from Clostridium cellulovorans: cellotetraose binding and cleavage. Acta Crystallogr F Struct Biol Commun. 2015 Oct 1;71(Pt 10):1264-72. doi:, 10.1107/S2053230X15015915. Epub 2015 Sep 23. PMID:26457517 doi:http://dx.doi.org/10.1107/S2053230X15015915
↑ Vincken JP, Beldman G, Voragen AG. Substrate specificity of endoglucanases: what determines xyloglucanase activity? Carbohydr Res. 1997 Mar 13;298(4):299-310. PMID:9098958
↑ Suyotha W, Yano S, Wakayama M. α-1,3-Glucanase: present situation and prospect of research. World J Microbiol Biotechnol. 2016 Feb;32(2):30. PMID:26748807 doi:10.1007/s11274-015-1977-0
↑ Kirubakaran SI, Sakthivel N. Cloning and overexpression of antifungal barley chitinase gene in Escherichia coli. Protein Expr Purif. 2007 Mar;52(1):159-66. PMID:17029984 doi:10.1016/j.pep.2006.08.012

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Michal Harel, Joel L. Sussman, Alexander Berchansky

[1] Barras DR, Stone BA. Beta-1,3-glucan hydrolases from Euglena gracilis. I. The nature of the hydrolases. Biochim Biophys Acta. 1969 Nov 4;191(2):329-41. PMID:5354264

[2] Tsai LC, Amiraslanov I, Chen HR, Chen YW, Lee HL, Liang PH, Liaw YC. Structures of exoglucanase from Clostridium cellulovorans: cellotetraose binding and cleavage. Acta Crystallogr F Struct Biol Commun. 2015 Oct 1;71(Pt 10):1264-72. doi:, 10.1107/S2053230X15015915. Epub 2015 Sep 23. PMID:26457517 doi:http://dx.doi.org/10.1107/S2053230X15015915

[3] Vincken JP, Beldman G, Voragen AG. Substrate specificity of endoglucanases: what determines xyloglucanase activity? Carbohydr Res. 1997 Mar 13;298(4):299-310. PMID:9098958

[4] Suyotha W, Yano S, Wakayama M. α-1,3-Glucanase: present situation and prospect of research. World J Microbiol Biotechnol. 2016 Feb;32(2):30. PMID:26748807 doi:10.1007/s11274-015-1977-0

[5] Kirubakaran SI, Sakthivel N. Cloning and overexpression of antifungal barley chitinase gene in Escherichia coli. Protein Expr Purif. 2007 Mar;52(1):159-66. PMID:17029984 doi:10.1016/j.pep.2006.08.012

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Glucanase

3D structures of glucanase

ReferencesReferences

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