Cutinase

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Cutinase (CUT) or α/β hydrolase family protein the conversion of cutin to cutin monomers. Cutin is a main component of the plant cuticle. Polymeric cutin which is composed of hydroxyl and hydroxyepoxy fatty acids is found in the outer layer of plants. CUT is found in fungi and bacteria. CUT degrades the cutin enabling pathogens’ penetration into plant cells. CUT is inhibited by organophosphates like E600 (diethyl-p-nitrophenyl phosphate) and organophosphonates.[1]

Relevance

Inhibition of CUT can prevent fungal plant infection[2].

Structural highlights

Upon CUT inhibition by organophosphates the .[3]

3D Structures of cutinase

Cutinase 3D structures


Structure of cutinase (PDB entry 4oyl)

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ReferencesReferences

  1. Longhi S, Czjzek M, Lamzin V, Nicolas A, Cambillau C. Atomic resolution (1.0 A) crystal structure of Fusarium solani cutinase: stereochemical analysis. J Mol Biol. 1997 May 16;268(4):779-99. PMID:9175860 doi:http://dx.doi.org/10.1006/jmbi.1997.1000
  2. Maiti IB, Kolattukudy PE. Prevention of fungal infection of plants by specific inhibition of cutinase. Science. 1979 Aug 3;205(4405):507-8. PMID:17758793 doi:http://dx.doi.org/10.1126/science.205.4405.507
  3. Kold D, Dauter Z, Laustsen AK, Brzozowski AM, Turkenburg JP, Nielsen AD, Koldso H, Petersen E, Schiott B, De Maria L, Wilson KS, Svendsen A, Wimmer R. Thermodynamic and structural investigation of the specific SDS binding of humicola insolens cutinase. Protein Sci. 2014 May 16. doi: 10.1002/pro.2489. PMID:24832484 doi:http://dx.doi.org/10.1002/pro.2489

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