8p5n

Arsenate reductase (ArsC2) from Deinococcus indicus, co-crystallized with arsenateArsenate reductase (ArsC2) from Deinococcus indicus, co-crystallized with arsenate

Structural highlights 8p5n is a 2 chain structure with sequence from Deinococcus indicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.5Å Ligands: , Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A246BSD0_9DEIO Publication Abstract from PubMed Arsenic (As) is a toxic heavy metal widely found in the environment that severely undermines the integrity of water resources. Bioremediation of toxic compounds is an appellative sustainable technology with a balanced cost-effective setup. To pave the way for the potential use of Deinococcus indicus, an arsenic resistant bacterium, as a platform for arsenic bioremediation, an extensive characterization of its resistance to cellular insults is paramount. A comparative analysis of D. indicus cells grown in two rich nutrient media conditions (M53 and TGY) revealed distinct resistance patterns when cells are subjected to stress via UV-C and methyl viologen (MV). Cells grown in M53 demonstrated higher resistance to both UV-C and MV. Moreover, cells grow to higher density upon exposure to 25 mM As(V) in M53 in comparison with TGY. This analysis is pivotal for the culture of microbial species in batch culture bioreactors for bioremediation purposes. We also demonstrate for the first time the presence of polyphosphate granules in D. indicus which are also found in a few Deinococcus species. To extend our analysis, we also characterized DiArsC2 (arsenate reductase) involved in arsenic detoxification and structurally determined different states, revealing the structural evidence for a catalytic cysteine triple redox system. These results contribute for our understanding into the D. indicus resistance mechanism against stress conditions. Unraveling the multifaceted resilience of arsenic resistant bacterium Deinococcus indicus.,Gouveia AG, Salgueiro BA, Ranmar DO, Antunes WDT, Kirchweger P, Golani O, Wolf SG, Elbaum M, Matias PM, Romao CV Front Microbiol. 2023 Aug 24;14:1240798. doi: 10.3389/fmicb.2023.1240798. , eCollection 2023. PMID:37692390 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 8p5n, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image