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ZjOGT3, flavonoid 7,4'-di-O-glycosyltransferaseZjOGT3, flavonoid 7,4'-di-O-glycosyltransferase
Structural highlights
FunctionPublication Abstract from PubMedA highly efficient and promiscuous 7,4'-di-O-glycosyltransferase ZjOGT3 was discovered from the medicinal plant Ziziphus jujuba var. spinosa. ZjOGT3 could sequentially catalyse 4'- and 7-O-glycosylation of flavones to produce 7,4'-di-O-glycosides with obvious regio-selectivity. For 7,4'-dihydroxyl flavanones and 3-O-glycosylated 7,4'-dihydroxyl flavones, ZjOGT3 selectively catalyses 7-O-glycosylation. The crystal structure of ZjOGT3 was solved. Structural analysis, DFT calculations, MD simulations, and site-directed mutagenesis reveal that the regio-selectivity is mainly controlled by the enzyme microenvironment for 7,4'-dihydroxyl flavones and 3-O-glycosylated 7,4'-dihydroxyl flavones. For 7,4'-dihydroxyl flavanones, the selectivity is mainly controlled by intrinsic reactivity. ZjOGT3 is the first plant flavonoid 7,4'-di-O-glycosyltransferase with a crystal structure. This work could help understand the catalytic mechanisms of multi-site glycosyltransferases and provides an efficient approach to synthesise O-glycosides with medicinal potential. Functional characterization, structural basis, and regio-selectivity control of a promiscuous flavonoid 7,4'-di-O-glycosyltransferase from Ziziphus jujuba var. spinosa.,Wang ZL, Wei W, Wang HD, Zhou JJ, Wang HT, Chen K, Wang RS, Li FD, Qiao X, Zhou H, Liang Y, Ye M Chem Sci. 2023 Mar 29;14(16):4418-4425. doi: 10.1039/d2sc06504e. eCollection 2023 , Apr 26. PMID:37123177[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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