8flj

Cas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNACas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNA

Structural highlights

8flj is a 14 chain structure with sequence from Pseudomonas aeruginosa PA14 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.48Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CAS1_PSEAB CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Involved in the integration of spacer DNA into the CRISPR cassette (By similarity). Acts as a ss- and dsDNA-specific endonuclease.^[1]

References

↑ Wiedenheft B, Zhou K, Jinek M, Coyle SM, Ma W, Doudna JA. Structural basis for DNase activity of a conserved protein implicated in CRISPR-mediated genome defense. Structure. 2009 Jun 10;17(6):904-12. PMID:19523907 doi:10.1016/j.str.2009.03.019

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OCA

[1] Wiedenheft B, Zhou K, Jinek M, Coyle SM, Ma W, Doudna JA. Structural basis for DNase activity of a conserved protein implicated in CRISPR-mediated genome defense. Structure. 2009 Jun 10;17(6):904-12. PMID:19523907 doi:10.1016/j.str.2009.03.019

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8flj

Cas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNACas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNA

Structural highlights

Function

References

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8flj

Cas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNACas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNA

Structural highlights

Function

References

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