8e1v

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Asp1 kinase in complex with ADPNP Mg IP6Asp1 kinase in complex with ADPNP Mg IP6

Structural highlights

8e1v is a 2 chain structure with sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VIP1_SCHPO Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. Phosphorylates inositol hexakisphosphate (InsP6) at position 1 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, phosphorylates PP-InsP5 at position 1, produced by IP6Ks from InsP6, to produce (PP)2-InsP4 (By similarity). Required for maintaining cellular integrity, normal growth and interactions with the ARP complex (PubMed:10388810). Acts as a regulator of the PHO80-PHO85 cyclin/cyclin-dependent kinase (CDK) complex, thereby regulating signaling of phosphate availability (By similarity). Required for the function of the cortical actin cytoskeleton, possibly by participating in correct F-actin localization and ensuring polarized growth (PubMed:10388810). Regulates polarized growth and modulates interphase microtubule cytoskeleton. Regulates microtubule dynamics without the requirement of microtubule plus-end tracking protein Mal3. Required for growth zone selection (PubMed:25254656).[UniProtKB:O43314][UniProtKB:Q06685][1] [2]

References

  1. Feoktistova A, McCollum D, Ohi R, Gould KL. Identification and characterization of Schizosaccharomyces pombe asp1(+), a gene that interacts with mutations in the Arp2/3 complex and actin. Genetics. 1999 Jul;152(3):895-908. doi: 10.1093/genetics/152.3.895. PMID:10388810 doi:http://dx.doi.org/10.1093/genetics/152.3.895
  2. Pohlmann J, Risse C, Seidel C, Pohlmann T, Jakopec V, Walla E, Ramrath P, Takeshita N, Baumann S, Feldbrugge M, Fischer R, Fleig U. The Vip1 inositol polyphosphate kinase family regulates polarized growth and modulates the microtubule cytoskeleton in fungi. PLoS Genet. 2014 Sep 25;10(9):e1004586. doi: 10.1371/journal.pgen.1004586. , eCollection 2014 Sep. PMID:25254656 doi:http://dx.doi.org/10.1371/journal.pgen.1004586

8e1v, resolution 1.90Å

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OCA