8cez

HK97 Portal Protein In situ (prohead II)HK97 Portal Protein In situ (prohead II)

Structural highlights 8cez is a 12 chain structure with sequence from Hendrixvirus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.97Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PORTL_BPHK7 Forms the portal vertex of the capsid. This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection. The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel. Binds to the terminase subunits to form the packaging machine.[UniProtKB:P25480] Publication Abstract from PubMed Double-stranded DNA viruses utilise machinery, made of terminase proteins, to package viral DNA into the capsid. For cos bacteriophage, a defined signal, recognised by small terminase, flanks each genome unit. Here we present the first structural data for a cos virus DNA packaging motor, assembled from the bacteriophage HK97 terminase proteins, procapsids encompassing the portal protein, and DNA containing a cos site. The cryo-EM structure is consistent with the packaging termination state adopted after DNA cleavage, with DNA density within the large terminase assembly ending abruptly at the portal protein entrance. Retention of the large terminase complex after cleavage of the short DNA substrate suggests that motor dissociation from the capsid requires headful pressure, in common with pac viruses. Interestingly, the clip domain of the 12-subunit portal protein does not adhere to C12 symmetry, indicating asymmetry induced by binding of the large terminase/DNA. The motor assembly is also highly asymmetric, showing a ring of 5 large terminase monomers, tilted against the portal. Variable degrees of extension between N- and C-terminal domains of individual subunits suggest a mechanism of DNA translocation driven by inter-domain contraction and relaxation. Insights into a viral motor: the structure of the HK97 packaging termination assembly.,Hawkins DEDP, Bayfield OW, Fung HKH, Grba DN, Huet A, Conway JF, Antson AA Nucleic Acids Res. 2023 Jul 21;51(13):7025-7035. doi: 10.1093/nar/gkad480. PMID:37293963 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Portal protein 3D structures

spinqualitylabelsall models 8cez, resolution 2.97Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image