Structural highlightsFunctionRBS_SYNE7 RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.[HAMAP-Rule:MF_00859][1] [2] Beta-carboxysome assembly initiates when soluble RuBisCO aggregates is condensed into a liquid matrix in a pre-carboxysome by the RbcS-like domains of probably both CcmM58 and CcmM35. CcmN interacts with the N-terminus of CcmM58, and then recruits the CcmK2 major shell protein via CcmN's encapsulation peptide. Shell formation requires CcmK proteins and CcmO. CcmL caps the otherwise elongated carboxysome. Once fully encapsulated carboxysomes are formed, they migrate within the cell probably via interactions with the cytoskeleton.[3] [4]
References
- ↑ Nishimura T, Yamaguchi O, Takatani N, Maeda S, Omata T. In vitro and in vivo analyses of the role of the carboxysomal β-type carbonic anhydrase of the cyanobacterium Synechococcus elongatus in carboxylation of ribulose-1,5-bisphosphate. Photosynth Res. 2014 Sep;121(2-3):151-7. PMID:24585024 doi:10.1007/s11120-014-9986-7
- ↑ Wang H, Yan X, Aigner H, Bracher A, Nguyen ND, Hee WY, Long BM, Price GD, Hartl FU, Hayer-Hartl M. Rubisco condensate formation by CcmM in beta-carboxysome biogenesis. Nature. 2019 Feb;566(7742):131-135. doi: 10.1038/s41586-019-0880-5. Epub 2019 Jan, 23. PMID:30675061 doi:http://dx.doi.org/10.1038/s41586-019-0880-5
- ↑ Cameron JC, Wilson SC, Bernstein SL, Kerfeld CA. Biogenesis of a bacterial organelle: the carboxysome assembly pathway. Cell. 2013 Nov 21;155(5):1131-40. doi: 10.1016/j.cell.2013.10.044. PMID:24267892 doi:http://dx.doi.org/10.1016/j.cell.2013.10.044
- ↑ Wang H, Yan X, Aigner H, Bracher A, Nguyen ND, Hee WY, Long BM, Price GD, Hartl FU, Hayer-Hartl M. Rubisco condensate formation by CcmM in beta-carboxysome biogenesis. Nature. 2019 Feb;566(7742):131-135. doi: 10.1038/s41586-019-0880-5. Epub 2019 Jan, 23. PMID:30675061 doi:http://dx.doi.org/10.1038/s41586-019-0880-5
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