7z1i

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Plant myrosinase TGG1 from Arabidopsis thalianaPlant myrosinase TGG1 from Arabidopsis thaliana

Structural highlights

7z1i is a 4 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.09Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BGL38_ARATH Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones. These toxic degradation products can deter insect herbivores. Seems to function in abscisic acid (ABA) and methyl jasmonate (MeJA) signaling in guard cells. Functionally redundant with TGG2. Hydrolyzes sinigrin and, with lower efficiency, p-nitrophenyl beta-D-glucoside.[1] [2] [3] [4]

References

  1. Stranger BE, Mitchell-Olds T. Nucleotide variation at the myrosinase-encoding locus, TGG1, and quantitative myrosinase enzyme activity variation in Arabidopsis thaliana. Mol Ecol. 2005 Jan;14(1):295-309. PMID:15643972 doi:10.1111/j.1365-294X.2004.02403.x
  2. Barth C, Jander G. Arabidopsis myrosinases TGG1 and TGG2 have redundant function in glucosinolate breakdown and insect defense. Plant J. 2006 May;46(4):549-62. PMID:16640593 doi:10.1111/j.1365-313X.2006.02716.x
  3. Islam MM, Tani C, Watanabe-Sugimoto M, Uraji M, Jahan MS, Masuda C, Nakamura Y, Mori IC, Murata Y. Myrosinases, TGG1 and TGG2, redundantly function in ABA and MeJA signaling in Arabidopsis guard cells. Plant Cell Physiol. 2009 Jun;50(6):1171-5. PMID:19433491 doi:10.1093/pcp/pcp066
  4. Andersson D, Chakrabarty R, Bejai S, Zhang J, Rask L, Meijer J. Myrosinases from root and leaves of Arabidopsis thaliana have different catalytic properties. Phytochemistry. 2009 Jul-Aug;70(11-12):1345-54. PMID:19703694 doi:10.1016/j.phytochem.2009.07.036

7z1i, resolution 3.09Å

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OCA