7ug6
Cryo-EM structure of pre-60S ribosomal subunit, unmethylated G2922Cryo-EM structure of pre-60S ribosomal subunit, unmethylated G2922
Structural highlights
FunctionPublication Abstract from PubMedRNA modifications are widespread in biology and abundant in ribosomal RNA. However, the importance of these modifications is not well understood. We show that methylation of a single nucleotide, in the catalytic center of the large subunit, gates ribosome assembly. Massively parallel mutational scanning of the essential nuclear GTPase Nog2 identified important interactions with rRNA, particularly with the 2'-O-methylated A-site base Gm2922. We found that methylation of G2922 is needed for assembly and efficient nuclear export of the large subunit. Critically, we identified single amino acid changes in Nog2 that completely bypass dependence on G2922 methylation and used cryoelectron microscopy to directly visualize how methylation flips Gm2922 into the active site channel of Nog2. This work demonstrates that a single RNA modification is a critical checkpoint in ribosome biogenesis, suggesting that such modifications can play an important role in regulation and assembly of macromolecular machines. A single 2'-O-methylation of ribosomal RNA gates assembly of a functional ribosome.,Yelland JN, Bravo JPK, Black JJ, Taylor DW, Johnson AW Nat Struct Mol Biol. 2023 Jan;30(1):91-98. doi: 10.1038/s41594-022-00891-8. Epub , 2022 Dec 19. PMID:36536102[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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