7qxm

Crystal structure of the Vibrio cholerae replicative helicase (DnaB)Crystal structure of the Vibrio cholerae replicative helicase (DnaB)

Structural highlights 7qxm is a 6 chain structure with sequence from Vibrio cholerae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.8Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A085R2T8_VIBCL Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.[ARBA:ARBA00003574][RuleBase:RU362085] Publication Abstract from PubMed To enable chromosomal replication, DNA is unwound by the ATPase molecular motor replicative helicase. The bacterial helicase DnaB is a ring-shaped homo-hexamer whose conformational dynamics are being studied through its different 3D structural states adopted along its functional cycle. Our findings describe a new crystal structure for the apo-DnaB from Vibrio cholerae, forming a planar hexamer with pseudo-symmetry, constituted by a trimer of dimers in which the C-terminal domains delimit a triskelion-shaped hole. This hexamer is labile and inactive. We suggest that it represents an intermediate state allowing the formation of the active NTP-bound hexamer from dimers. The apo-form of the Vibrio cholerae replicative helicase DnaB is a labile and inactive planar trimer of dimers.,Cargemel C, Walbott H, Durand D, Legrand P, Ouldali M, Ferat JL, Marsin S, Quevillon-Cheruel S FEBS Lett. 2022 May 14. doi: 10.1002/1873-3468.14403. PMID:35568982 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Helicase 3D structures

spinqualitylabelsall models 7qxm, resolution 3.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image